Clinical significance of cell proliferation, microvessel density, and CD44 adhesion molecule expression in renal cell carcinoma

Nathalie Rioux-Leclercq, Jonathan Ira Epstein, Jean Yves Bansard, Bruno Turlin, Jean Jacques Patard, Andra Manunta, Theresa Chan, Marie Paule Ramee, Bernard Lobel, Jacques Philippe Moulinoux

Research output: Contribution to journalArticle

Abstract

Renal cell carcinoma (RCC) is known to have a wide variation in clinical outcome despite the use of conventional prognostic factors, such as staging or grading. A better knowledge of the biologic aggressiveness of RCC could facilitate the selection of patients at high risk of tumor progression. The aim of this study was to determine if use of measurements of vascular density, cell proliferation, and cell adhesion could better predict the biologic behavior of RCC. We immunohistochemically analyzed CD34, Ki-67, and CD44H expression on formalin-fixed, paraffin-embedded tissues from 73 RCCs for quantifying microvessel density (MVD), Ki-67 labeling index (LI), and CD44H LI, respectively. Univariate cancer-specific survival analysis showed that tumor stage (P <.01), tumor size (P <.001), nuclear grade (P <.01), metastasis (P <.001), MVD (P <.03), Ki-67 LI (P <.001), and CD44H LI (P <.0001) were predictors of tumor-related death. There was a statistical correlation between CD44H LI and both Ki-67 LI (r' = .3) and MVD (r' = -.44). Ki-67 LI (P <.04) and CD44H LI (P <.02), as well as metastasis (P <.008), emerged as independent predictors of cancer-specific survival in multivariate analysis in patients with metastases (P <.04 and P <.02, respectively) and in patients without metastases (P <.006 and P <.00001, respectively). Our study suggests that vascular density, cell proliferation, and cell adhesion represent a complex tumor-host interaction that may favor progression of RCC. Cell proliferation and CD44H expression appear to be powerful markers to identify patients with an adverse prognosis.

Original languageEnglish (US)
Pages (from-to)1209-1215
Number of pages7
JournalHuman Pathology
Volume32
Issue number11
DOIs
StatePublished - 2001

Fingerprint

Microvessels
Renal Cell Carcinoma
Cell Proliferation
Neoplasms
Neoplasm Metastasis
Cell Adhesion
Blood Vessels
Survival Analysis
Paraffin
Patient Selection
Formaldehyde
Multivariate Analysis
Survival

Keywords

  • CD34
  • CD44
  • Immunohistochemistry
  • Ki-67
  • Prognosis
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Clinical significance of cell proliferation, microvessel density, and CD44 adhesion molecule expression in renal cell carcinoma. / Rioux-Leclercq, Nathalie; Epstein, Jonathan Ira; Bansard, Jean Yves; Turlin, Bruno; Patard, Jean Jacques; Manunta, Andra; Chan, Theresa; Ramee, Marie Paule; Lobel, Bernard; Moulinoux, Jacques Philippe.

In: Human Pathology, Vol. 32, No. 11, 2001, p. 1209-1215.

Research output: Contribution to journalArticle

Rioux-Leclercq, N, Epstein, JI, Bansard, JY, Turlin, B, Patard, JJ, Manunta, A, Chan, T, Ramee, MP, Lobel, B & Moulinoux, JP 2001, 'Clinical significance of cell proliferation, microvessel density, and CD44 adhesion molecule expression in renal cell carcinoma', Human Pathology, vol. 32, no. 11, pp. 1209-1215. https://doi.org/10.1053/hupa.2001.28957
Rioux-Leclercq, Nathalie ; Epstein, Jonathan Ira ; Bansard, Jean Yves ; Turlin, Bruno ; Patard, Jean Jacques ; Manunta, Andra ; Chan, Theresa ; Ramee, Marie Paule ; Lobel, Bernard ; Moulinoux, Jacques Philippe. / Clinical significance of cell proliferation, microvessel density, and CD44 adhesion molecule expression in renal cell carcinoma. In: Human Pathology. 2001 ; Vol. 32, No. 11. pp. 1209-1215.
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T1 - Clinical significance of cell proliferation, microvessel density, and CD44 adhesion molecule expression in renal cell carcinoma

AU - Rioux-Leclercq, Nathalie

AU - Epstein, Jonathan Ira

AU - Bansard, Jean Yves

AU - Turlin, Bruno

AU - Patard, Jean Jacques

AU - Manunta, Andra

AU - Chan, Theresa

AU - Ramee, Marie Paule

AU - Lobel, Bernard

AU - Moulinoux, Jacques Philippe

PY - 2001

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N2 - Renal cell carcinoma (RCC) is known to have a wide variation in clinical outcome despite the use of conventional prognostic factors, such as staging or grading. A better knowledge of the biologic aggressiveness of RCC could facilitate the selection of patients at high risk of tumor progression. The aim of this study was to determine if use of measurements of vascular density, cell proliferation, and cell adhesion could better predict the biologic behavior of RCC. We immunohistochemically analyzed CD34, Ki-67, and CD44H expression on formalin-fixed, paraffin-embedded tissues from 73 RCCs for quantifying microvessel density (MVD), Ki-67 labeling index (LI), and CD44H LI, respectively. Univariate cancer-specific survival analysis showed that tumor stage (P <.01), tumor size (P <.001), nuclear grade (P <.01), metastasis (P <.001), MVD (P <.03), Ki-67 LI (P <.001), and CD44H LI (P <.0001) were predictors of tumor-related death. There was a statistical correlation between CD44H LI and both Ki-67 LI (r' = .3) and MVD (r' = -.44). Ki-67 LI (P <.04) and CD44H LI (P <.02), as well as metastasis (P <.008), emerged as independent predictors of cancer-specific survival in multivariate analysis in patients with metastases (P <.04 and P <.02, respectively) and in patients without metastases (P <.006 and P <.00001, respectively). Our study suggests that vascular density, cell proliferation, and cell adhesion represent a complex tumor-host interaction that may favor progression of RCC. Cell proliferation and CD44H expression appear to be powerful markers to identify patients with an adverse prognosis.

AB - Renal cell carcinoma (RCC) is known to have a wide variation in clinical outcome despite the use of conventional prognostic factors, such as staging or grading. A better knowledge of the biologic aggressiveness of RCC could facilitate the selection of patients at high risk of tumor progression. The aim of this study was to determine if use of measurements of vascular density, cell proliferation, and cell adhesion could better predict the biologic behavior of RCC. We immunohistochemically analyzed CD34, Ki-67, and CD44H expression on formalin-fixed, paraffin-embedded tissues from 73 RCCs for quantifying microvessel density (MVD), Ki-67 labeling index (LI), and CD44H LI, respectively. Univariate cancer-specific survival analysis showed that tumor stage (P <.01), tumor size (P <.001), nuclear grade (P <.01), metastasis (P <.001), MVD (P <.03), Ki-67 LI (P <.001), and CD44H LI (P <.0001) were predictors of tumor-related death. There was a statistical correlation between CD44H LI and both Ki-67 LI (r' = .3) and MVD (r' = -.44). Ki-67 LI (P <.04) and CD44H LI (P <.02), as well as metastasis (P <.008), emerged as independent predictors of cancer-specific survival in multivariate analysis in patients with metastases (P <.04 and P <.02, respectively) and in patients without metastases (P <.006 and P <.00001, respectively). Our study suggests that vascular density, cell proliferation, and cell adhesion represent a complex tumor-host interaction that may favor progression of RCC. Cell proliferation and CD44H expression appear to be powerful markers to identify patients with an adverse prognosis.

KW - CD34

KW - CD44

KW - Immunohistochemistry

KW - Ki-67

KW - Prognosis

KW - Renal cell carcinoma

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