Clinical scale rapid expansion of lymphocytes for adoptive cell transfer therapy in the WAVE ® bioreactor

Robert P.T. Somerville, Laura Devillier, Maria R. Parkhurst, Steven A. Rosenberg, Mark E. Dudley

Research output: Contribution to journalArticlepeer-review

Abstract

Background: To simplify clinical scale lymphocyte expansions, we investigated the use of the WAVE ®, a closed system bioreactor that utilizes active perfusion to generate high cell numbers in minimal volumes.Methods: We have developed an optimized rapid expansion protocol for the WAVE bioreactor that produces clinically relevant numbers of cells for our adoptive cell transfer clinical protocols.Results: TIL and genetically modified PBL were rapidly expanded to clinically relevant scales in both static bags and the WAVE bioreactor. Both bioreactors produced comparable numbers of cells; however the cultures generated in the WAVE bioreactor had a higher percentage of CD4+ cells and had a less activated phenotype.Conclusions: The WAVE bioreactor simplifies the process of rapidly expanding tumor reactive lymphocytes under GMP conditions, and provides an alternate approach to cell generation for ACT protocols.

Original languageEnglish (US)
Article number69
JournalJournal of translational medicine
Volume10
Issue number1
DOIs
StatePublished - Apr 4 2012

Keywords

  • Adoptive immunotherapy
  • Human
  • T cell receptors
  • T cells
  • Tumor immunity

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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