Clinical relevance and igg subclass determination of Non-HLA antibodies identified using endothelial cell precursors isolated from donor blood

Annette Jackson, Donna P. Lucas, J. Keith Melancon, Niraj M Desai

Research output: Contribution to journalArticle

Abstract

Background: ABO and human leukocyte antigen (HLA) alloantibodies provide major immunologic barriers to successful transplantation; however, there is increasing recognition for the role of anti-endothelial cell antibodies (AECAs) in allograft rejection. We investigated the relationship between AECAs identified using donor-derived endothelial cell precursors (ECPs) and kidney allograft rejection and function. Methods: Sixty live donor kidney recipients were tested pretransplant for AECAs and HLA-antibodies using flow cytometric crossmatch tests and solid-phase bead immunoassays. Renal allograft function was assessed by serum creatinine (SCr) values collected at early (mean, 50 days) and late (mean, 815 days) time points posttransplant and by incidence and type of rejection. Immunoglobulin G (IgG) subtype determination of both AECAs and HLA antibodies bound to ECPs was performed using flow cytometry. Results: Fourteen patients (23%) tested positive for donor-reactive IgG AECAs and had statistically higher SCr values and incidences of cellular rejection early posttransplant compared with 46 patients who tested negative (P=0.014 and P

Original languageEnglish (US)
Pages (from-to)54-60
Number of pages7
JournalTransplantation
Volume92
Issue number1
DOIs
StatePublished - Jul 15 2011

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Keywords

  • Alloantibodies
  • Endothelial cell
  • Kidney transplantation
  • Non-HLA antibodies
  • Rejection

ASJC Scopus subject areas

  • Transplantation

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