Clinical performance of non-radioactive assays for HIV-1 DNA amplified by the polymerase chain reaction

J. M. Wages, M. Hamdallah, A. K. Fowler, C. N. Roberts, R. R. Redfield, D. S. Burke

Research output: Contribution to journalArticlepeer-review

Abstract

A major factor preventing more widespread use of polymerase chain reaction in the clinical laboratory is the lack of convenient non-radioactive probe hybridization procedures which do not sacrifice sensitivity or specificity. In this report, we describe comparisons of probes labelled with biotin, digoxygenin, alkaline phosphatase, and32P. We report the comparison of solution or liquid hybridization assay and Southern blotting with digoxygenin-labelled oligonucleotides on a total of 64 clinical specimens. Perfect diagnostic agreement between the32P and digoxygenin probes was obtained. These data suggest that the non-radioactive assay as described is as sensitive and as specific as the assay with32P-Iabelled probes.

Original languageEnglish (US)
Pages (from-to)102-107
Number of pages6
JournalWorld Journal of Microbiology & Biotechnology
Volume9
Issue number1
DOIs
StatePublished - Jan 1993
Externally publishedYes

Keywords

  • HIV-1
  • non-radioactive DNA probes
  • polymerase chain reaction

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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