Clinical implementation of a procedure to prepare bone marrow cells from cadaveric vertebral bodies

Albert D. Donnenberg, Vijay S. Gorantla, Stefan Schneeberger, Linda R. Moore, Gerald Brandacher, Heather M. Stanczak, Eileen K. Koch, Wp Andrew Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Aims: Vertebral bone marrow is a rich and easily accessible source of hematopoietic and mesenchymal stem cells that has been used to promote chimerism and transplantation tolerance in connection with cadaveric organ transplantation. The purpose of this study is to provide a detailed account of the procedure used to prepare the first five vertebral bone marrow products for infusion in conjunction with the first hand/hand-forelimb transplants performed at the University of Pittsburgh (PA, USA). Materials & methods: The cell separation and release testing were performed at the University of Pittsburgh Cancer Institutes Hematopoietic Stem Cell Laboratory, a Good Manufacturing Practice-compliant facility accredited for clinical cell processing by the Foundation for Accreditation of Cellular Therapy (FACT) and for clinical flow cytometry by the College of American Pathologists (CAP).

Original languageEnglish (US)
Pages (from-to)701-706
Number of pages6
JournalRegenerative Medicine
Volume6
Issue number6
DOIs
StatePublished - Nov 2011

Keywords

  • Pittsburgh Protocol
  • composite organ transplantation
  • good manufacturing practices
  • hand transplantation
  • vertebral bodies
  • vertebral bone marrow

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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