Clinical findings and pro-inflammatory cytokines in dengue patients in Western India: A facility-based study

D. Priyadarshini, Rajesh R. Gadia, Anuradha Tripathy, K. R. Gurukumar, Asha Bhagat, Sampada Patwardhan, Nitin Mokashi, Dhananjay Vaidya, Paresh S. Shah, D. Cecilia

Research output: Contribution to journalArticle

Abstract

Background:Descriptions of dengue immunopathogenesis have largely relied on data from South-east Asia and America, while India is poorly represented. This study characterizes dengue cases from Pune, Western India, with respect to clinical profile and pro-inflammatory cytokines. Methodology/Principal Findings:In 2005, 372 clinically suspected dengue cases were tested by MAC-ELISA and RT-PCR for dengue virus (DENV) aetiology. The clinical profile was recorded at the hospital. Circulating levels of IFN-γ, TNF-α, IL-6, and IL-8 were assessed by ELISA and secondary infections were defined by IgM to IgG ratio. Statistical analysis was carried out using the SPSS 11.0 version. Of the 372 individuals, 221 were confirmed to be dengue cases. Three serotypes, DENV-1, 2 and 3 were co-circulating and one case of dual infection was identified. Of 221 cases, 159 presented with Dengue fever (DF) and 62 with Dengue hemorrhagic fever (DHF) of which six had severe DHF and one died of shock. There was a strong association of rash, abdominal pain and conjunctival congestion with DHF. Levels of IFN-γ were higher in DF whereas IL-6 and IL-8 were higher in DHF cases (p

Original languageEnglish (US)
Article numbere8709
JournalPLoS One
Volume5
Issue number1
DOIs
StatePublished - Jan 14 2010

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dengue
Dengue
Severe Dengue
Interleukin-8
Viruses
India
Interleukin-6
cytokines
Cytokines
Immunoglobulin M
Dengue virus
Statistical methods
Dengue Virus
Immunoglobulin G
interleukin-8
interleukin-6
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Far East
Exanthema

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Priyadarshini, D., Gadia, R. R., Tripathy, A., Gurukumar, K. R., Bhagat, A., Patwardhan, S., ... Cecilia, D. (2010). Clinical findings and pro-inflammatory cytokines in dengue patients in Western India: A facility-based study. PLoS One, 5(1), [e8709]. https://doi.org/10.1371/journal.pone.0008709

Clinical findings and pro-inflammatory cytokines in dengue patients in Western India : A facility-based study. / Priyadarshini, D.; Gadia, Rajesh R.; Tripathy, Anuradha; Gurukumar, K. R.; Bhagat, Asha; Patwardhan, Sampada; Mokashi, Nitin; Vaidya, Dhananjay; Shah, Paresh S.; Cecilia, D.

In: PLoS One, Vol. 5, No. 1, e8709, 14.01.2010.

Research output: Contribution to journalArticle

Priyadarshini, D, Gadia, RR, Tripathy, A, Gurukumar, KR, Bhagat, A, Patwardhan, S, Mokashi, N, Vaidya, D, Shah, PS & Cecilia, D 2010, 'Clinical findings and pro-inflammatory cytokines in dengue patients in Western India: A facility-based study', PLoS One, vol. 5, no. 1, e8709. https://doi.org/10.1371/journal.pone.0008709
Priyadarshini D, Gadia RR, Tripathy A, Gurukumar KR, Bhagat A, Patwardhan S et al. Clinical findings and pro-inflammatory cytokines in dengue patients in Western India: A facility-based study. PLoS One. 2010 Jan 14;5(1). e8709. https://doi.org/10.1371/journal.pone.0008709
Priyadarshini, D. ; Gadia, Rajesh R. ; Tripathy, Anuradha ; Gurukumar, K. R. ; Bhagat, Asha ; Patwardhan, Sampada ; Mokashi, Nitin ; Vaidya, Dhananjay ; Shah, Paresh S. ; Cecilia, D. / Clinical findings and pro-inflammatory cytokines in dengue patients in Western India : A facility-based study. In: PLoS One. 2010 ; Vol. 5, No. 1.
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