Clinical evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to isoniazid or rifampin

Fernanda C.Q. Mello, Mayra S. Arias, Senia Rosales, Anna Grazia Marsico, Ada Pavón, Carlos Alvarado-Gâlvez, Carlos Leonardo Carvalho Pessôa, Melly Pérez, Monica K. Andrade, Afranio L. Kritski, Leila S. Fonseca, Richard E. Chaisson, Michael E. Kimerling, Susan E. Dorman

Research output: Contribution to journalArticle

Abstract

This prospective study evaluated the performance of the microscopic observation drug susceptibility (MODS) assay for the direct detection of Mycobacterium tuberculosis drug resistance. MODS assay sensitivity, specificity, and positive and negative predictive values were 96.7% (95% confidence interval [95% CI], 92.1 to 98.8%), 78.4% (95% CI, 73.5 to 80.6%), 82.4% (95% CI, 78.4 to 84.2%), and 95.8% (95% CI, 89.9 to 98.5%), respectively, for isoniazid resistance and 96.0% (95% CI, 90.3 to 98.6%), 82.9% (95% CI, 78.8 to 84.7%), 80.0% (95% CI, 75.2 to 82.1%), and 96.7% (95% CI, 91.9 to 98.8%), respectively, for rifampin resistance. For both rifampin and isoniazid testing, the likelihood ratio for a negative test was ≤0.05, indicating that the MODS assay may be useful for ruling out drug resistance.

Original languageEnglish (US)
Pages (from-to)3387-3389
Number of pages3
JournalJournal of clinical microbiology
Volume45
Issue number10
DOIs
StatePublished - Oct 2007

ASJC Scopus subject areas

  • Microbiology (medical)

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    Mello, F. C. Q., Arias, M. S., Rosales, S., Marsico, A. G., Pavón, A., Alvarado-Gâlvez, C., Pessôa, C. L. C., Pérez, M., Andrade, M. K., Kritski, A. L., Fonseca, L. S., Chaisson, R. E., Kimerling, M. E., & Dorman, S. E. (2007). Clinical evaluation of the microscopic observation drug susceptibility assay for detection of Mycobacterium tuberculosis resistance to isoniazid or rifampin. Journal of clinical microbiology, 45(10), 3387-3389. https://doi.org/10.1128/JCM.00580-07