Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus

Emil P. Miskovsky, Anthony V. Carrella, Karen Gutekunst, Chien An Sun, Thomas C Quinn, David L Thomas

Research output: Contribution to journalArticle

Abstract

Rational clinical application of quantitative assessments of hepatitis C virus (HCV) RNA depends on an understanding of factors affecting the assay and its intrinsic variability. The effects of three types of blood collection tubes, two storage temperatures, five processing times, and two laboratories on a commercially available quantitative reverse transcriptase PCR assay (AMPLICOR HCV MONITOR) were evaluated. HCV RNA concentrations were assessed in 356 specimens representing 178 aliquots from nine patients. In a multivariate generalized linear model, HCV RNA concentrations decreased when centrifugation was delayed more than 6 h (P = 0.005) and were marginally different between laboratories (P = 0.06), but precentrifugation storage temperature (P = 1.00) and anticoagulation (P = 0.22) had no effect. After adjusting for other factors, the HCV concentration of 95% of a subject's samples were within 0.44 log. Specimens procured for reverse transcriptase PCR-based quantitative HCV testing should be centrifuged within 6 h of collection. Serial assessments should ideally be performed in the same laboratory, and changes in HCV RNA concentration of less than 0.44 log may not be biologically important.

Original languageEnglish (US)
Pages (from-to)1975-1979
Number of pages5
JournalJournal of Clinical Microbiology
Volume34
Issue number8
StatePublished - Aug 1996

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Hepacivirus
Polymerase Chain Reaction
RNA
Reverse Transcriptase Polymerase Chain Reaction
Temperature
Centrifugation
Linear Models

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus. / Miskovsky, Emil P.; Carrella, Anthony V.; Gutekunst, Karen; Sun, Chien An; Quinn, Thomas C; Thomas, David L.

In: Journal of Clinical Microbiology, Vol. 34, No. 8, 08.1996, p. 1975-1979.

Research output: Contribution to journalArticle

Miskovsky, Emil P. ; Carrella, Anthony V. ; Gutekunst, Karen ; Sun, Chien An ; Quinn, Thomas C ; Thomas, David L. / Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus. In: Journal of Clinical Microbiology. 1996 ; Vol. 34, No. 8. pp. 1975-1979.
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