Clinical application of novel sample processing technology for the identification of salmonellae by using DNA probes

D. R. School, C. Kaufmann, J. D. Jollick, C. K. York, G. R. Goodrum, P. Charache

Research output: Contribution to journalArticle

Abstract

Two hundred and fifty clinical fecal specimens collected over a 7-month period were analyzed for the presence of salmonellae by a rapid DNA hybridization procedure. Hybridizations were performed by using a novel specimen processing protocol called wicking and a previously unreported 1,600-base-pair probe cloned from Salmonella enteritidis DNA. The probe was shown to be reactive with all 70 Salmonella serotypes tested and not reactive with 101 stock strains of other enteric bacteria. Southern analysis of 30 Salmonella isolates representing 22 serotypes suggested that the probe sequence was highly conserved, appearing as a 1,600-base-pair band in a Bg/II digest of isolate DNA in 29 of 30 isolates and as a 2,300-base-pair fragement in 1 of the isolates. The probe correctly identified all salmonellae (nine isolates) among 47 H2S-producing colonies tested from among 250 clinical specimens cultured on xylose-lysine-desoxycholate medium. Salmonellae grown on xylose-lysine-desoxycholate medium gave consistently higher hybridization values than did those grown on either MacConkey or Hektoen enteric agar. In addition, of eight gram-negative broth enrichments in which salmonellae were identified by conventional means, seven were probe positive. The use of this nucleic acid probe and hybridization technique provides a simple and rapid identification of Salmonella species.

Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalJournal of Clinical Microbiology
Volume28
Issue number2
Publication statusPublished - 1990
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

School, D. R., Kaufmann, C., Jollick, J. D., York, C. K., Goodrum, G. R., & Charache, P. (1990). Clinical application of novel sample processing technology for the identification of salmonellae by using DNA probes. Journal of Clinical Microbiology, 28(2), 237-241.