Clindamycin-induced enterocolitis in hamsters as a model of pseudomembranous colitis in patients

T. W. Chang, J. G. Bartlett, S. L. Gorbach, A. B. Onderdonk

Research output: Contribution to journalArticlepeer-review

Abstract

Stools from a patient with antibiotic-associated colitis and cecal contents from a hamster with clindamycin-induced enterocolitis were compared in cytotoxicity assay to determine common properties. Both specimens produced actinomorphic changes in human amnion cells at 10-7 dilutions. The toxin was acid labile, heat labile, nonether extractable, non-dialyzable, and produced maximum activity at 60% with ammonium sulfate precipitation. Cytotoxicity was neutralized with clostridial antitoxin but not with equine serum. Clostridium difficile was recovered in high concentrations in specimens from both the hamster and patient. The supernatants of these C. difficile strains produced cytotoxic effects which were also neutralized by clostridial antitoxins. These results indicate that clindamycin-induced enterocolitis in hamsters is a model of human disease and implicate toxin-producing clostridia as responsible agents.

Original languageEnglish (US)
Pages (from-to)526-529
Number of pages4
JournalInfection and immunity
Volume20
Issue number2
DOIs
StatePublished - 1978
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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