ClC-5: Ontogeny of an alternative chloride channel in respiratory epithelia

Rebecca D. Edmonds, Ian V. Silva, William B. Guggino, Robert B. Butler, Pamela L. Zeitlin, Carol J. Blaisdell

Research output: Contribution to journalArticlepeer-review

Abstract

Chloride transport is critical to many functions of the lung. Molecular defects in the best-known chloride channel, cystic fibrosis transmembrane conductance regulator (CFTR), lead to impaired function of airway defensins, hydration of airway surface fluid, and mucociliary clearance leading to chronic lung disease, and premature death, but do not cause defects in lung development. We examined the expression of one member of the ClC family of volume- and voltage-regulated channels using the ribonuclease protection assay and Western blot analysis in rats. ClC-5 mRNA and protein are most strongly expressed in the fetal lung, and expression is maintained although down-regulated postnatally. In addition, using immunocytochemistry, we find that ClC-5 is predominantly expressed along the luminal surface of the airway epithelium, suggesting that ClC-5 may participate in lung chloride secretion. Identifying candidate genes for critical ion transport functions is essential for understanding normal lung morphogenesis and the pathophysiology of several lung diseases. In addition, the manipulation of non-CFTR chloride channels may provide a viable approach for treating cystic fibrosis lung disease.

Original languageEnglish (US)
Pages (from-to)L501-L507
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume282
Issue number3 26-3
DOIs
StatePublished - 2002

Keywords

  • Calcium channel family
  • Developmental regulation
  • Ion channels

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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