Classification of mouse B cell types using surfaceome proteotype maps

Marc van Oostrum, Maik Müller, Fabian Klein, Roland Bruderer, Hui Zhang, Patrick G.A. Pedrioli, Lukas Reiter, Panagiotis Tsapogas, Antonius Rolink, Bernd Wollscheid

Research output: Contribution to journalArticlepeer-review

Abstract

System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.

Original languageEnglish (US)
Article number5734
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

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