TY - JOUR
T1 - Classification of mouse B cell types using surfaceome proteotype maps
AU - van Oostrum, Marc
AU - Müller, Maik
AU - Klein, Fabian
AU - Bruderer, Roland
AU - Zhang, Hui
AU - Pedrioli, Patrick G.A.
AU - Reiter, Lukas
AU - Tsapogas, Panagiotis
AU - Rolink, Antonius
AU - Wollscheid, Bernd
N1 - Funding Information:
We acknowledge A. Leitner and R. Aebersold for sharing and maintaining their instrumentation. We are grateful to the members of the R.A. and B.W. research groups for suggestions and support at all stages of the project. We thank K. Novy for facilitating collaboration, J. R. Wyatt for text editing, J. Niebel and S. Sung for technical and T. Splettstoeser for graphical support. This work benefited from data assembled by the Immgen consortium. The following agencies are thanked for funding: ETH (grant ETH-30 17-1 and grant ETH-25 15-2) and Swiss National Science Foundation (grant 31003A_160259) for B.W. A.R. was holder of a chair in immunology endowed by L. Hoffman-La Roche Ltd, Basel.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
AB - System-wide quantification of the cell surface proteotype and identification of extracellular glycosylation sites is challenging when samples are limited. Here, we miniaturize and automate the previously described Cell Surface Capture (CSC) technology, increasing sensitivity, reproducibility and throughput. We use this technology, which we call autoCSC, to create population-specific surfaceome maps of developing mouse B cells and use targeted flow cytometry to uncover developmental cell subpopulations.
UR - http://www.scopus.com/inward/record.url?scp=85076623725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076623725&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-13418-5
DO - 10.1038/s41467-019-13418-5
M3 - Article
C2 - 31844046
AN - SCOPUS:85076623725
VL - 10
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 5734
ER -