Classification, averaging and reconstruction of macromolecules in electron tomography

A. Bartesaghi, P. Sprechmann, G. Randall, G. Sapiro, S. Subramaniam

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Electron tomography provides opportunities to determine diree-dimensional cellular architecture at resolutions high enough to identify individual macromolecules such as proteins. Image analysis of such data poses a challenging problem due to the extremely low signal-to-noise ratios that makes individual volumes simply too noisy to allow reliable structural interpretation. This requires using averaging techniques to boost the signal-to-noise ratios, a common practice in electron microscopy single particle analysis where they have proven to be very powerful in elucidating high resolution structure. Although there are significant similarities in the way data is processed, several new problems arise in the tomography case that have to be properly dealt with. Such problems involve dealing with the missing wedge characteristic of limited angle tomography, the need for robust and efficient 3D alignment routines, and design of mediods that account for diverse conformations through the use of classification. We present a framework for reconstruction via alignment, classification and averaging of volumes obtained from limited angle electron tomography, providing a powerful tool for high resolution structure determination and description of conformational variability in a biological context.

Original languageEnglish (US)
Title of host publication2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings
Pages244-247
Number of pages4
DOIs
StatePublished - 2007
Externally publishedYes
Event2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07 - Arlington, VA, United States
Duration: Apr 12 2007Apr 15 2007

Other

Other2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro; ISBI'07
CountryUnited States
CityArlington, VA
Period4/12/074/15/07

Keywords

  • Clustering methods
  • Image classification
  • Image registration
  • Tomography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Medicine(all)

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  • Cite this

    Bartesaghi, A., Sprechmann, P., Randall, G., Sapiro, G., & Subramaniam, S. (2007). Classification, averaging and reconstruction of macromolecules in electron tomography. In 2007 4th IEEE International Symposium on Biomedical Imaging: From Nano to Macro - Proceedings (pp. 244-247). [4193268] https://doi.org/10.1109/ISBI.2007.356834