Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway

Teclise Ng, Jae Ryun Ryu, Jae Ho Sohn, Terence Tan, Hongjun Song, Guo li Ming, Eyleen L K Goh

Research output: Contribution to journalArticle

Abstract

Class 3 semaphorins are well-known axonal guidance cues during the embryonic development of mammalian nervous system. However, their activity on postnatally differentiated neurons in neurogenic regions of adult brains has not been characterized. We found that silencing of semaphorin receptors neuropilins (NRP) 1 or 2 in neural progenitors at the adult mouse dentate gyrus resulted in newly differentiated neurons with shorter dendrites and simpler branching in vivo. Tyrosine phosphorylation (Tyr 397) and serine phosphorylation (Ser 732) of FAK were essential for these effects. Semaphorin 3A and 3F mediate serine phosphorylation of FAK through the activation of Cdk5. Silencing of either Cdk5 or FAK in newborn neurons phenocopied the defects in dendritic development seen upon silencing of NRP1 or NRP2. Furthermore, in vivo overexpression of Cdk5 or FAK rescued the dendritic phenotypes seen in NRP1 and NRP2 deficient neurons. These results point to a novel role for class 3 semaphorins in promoting dendritic growth and branching during adult hippocampal neurogenesis through the activation of Cdk5-FAK signaling pathway.

Original languageEnglish (US)
Article numbere65572
JournalPLoS One
Volume8
Issue number6
DOIs
StatePublished - Jun 10 2013

Fingerprint

Semaphorins
dendrites
Dendrites
Phosphorylation
Neurons
neonates
neurons
Newborn Infant
phosphorylation
Growth
serine
Serine
branching
Neuropilin-2
Chemical activation
Semaphorin-3A
Neuropilin-1
neurogenesis
Neurogenesis
Dentate Gyrus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ng, T., Ryu, J. R., Sohn, J. H., Tan, T., Song, H., Ming, G. L., & Goh, E. L. K. (2013). Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway. PLoS One, 8(6), [e65572]. https://doi.org/10.1371/journal.pone.0065572

Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway. / Ng, Teclise; Ryu, Jae Ryun; Sohn, Jae Ho; Tan, Terence; Song, Hongjun; Ming, Guo li; Goh, Eyleen L K.

In: PLoS One, Vol. 8, No. 6, e65572, 10.06.2013.

Research output: Contribution to journalArticle

Ng, T, Ryu, JR, Sohn, JH, Tan, T, Song, H, Ming, GL & Goh, ELK 2013, 'Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway', PLoS One, vol. 8, no. 6, e65572. https://doi.org/10.1371/journal.pone.0065572
Ng, Teclise ; Ryu, Jae Ryun ; Sohn, Jae Ho ; Tan, Terence ; Song, Hongjun ; Ming, Guo li ; Goh, Eyleen L K. / Class 3 Semaphorin Mediates Dendrite Growth in Adult Newborn Neurons through Cdk5/FAK Pathway. In: PLoS One. 2013 ; Vol. 8, No. 6.
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