Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium

Karen Byer, Saeed R. Khan

Research output: Contribution to journalArticle

Abstract

Purpose: Oxalate and calcium oxalate (CaOx) crystals are injurious to renal epithelial cells. The injury is caused by the production of reactive oxygen species (ROS). Citrate is a well-known inhibitor of CaOx crystallization and as such it is one of the major therapeutic agents prescribed. Since citrate increases cellular reduced nicotinamide adenine dinucleotide phosphate and glutathione (GSH), we hypothesized that exogenously administered citrate should act as an anti-oxidant and protect cells from oxalate induced injury. Materials and Methods: We exposed LLC-PK1 and MDCK cells to 500 νM/ml oxalate or 150 μg/cm2 calcium oxalate crystals for 30, 60 and 180 minutes with or without 3 mg/ml citrate in the medium. We determined cell viability by lactate dehydrogenase release and trypan blue exclusion, ROS involvement by changes in hydrogen peroxide and GSH, and lipid peroxidation by quantifying 8-isoprostane. Results: The presence of citrate was associated with significant decrease in lactate dehydrogenase release (p

Original languageEnglish (US)
Pages (from-to)640-646
Number of pages7
JournalJournal of Urology
Volume173
Issue number2
DOIs
StatePublished - Feb 2005
Externally publishedYes

Fingerprint

Calcium Oxalate
Oxalates
Citric Acid
Epithelium
Kidney
8-epi-prostaglandin F2alpha
L-Lactate Dehydrogenase
Reactive Oxygen Species
LLC-PK1 Cells
Madin Darby Canine Kidney Cells
Trypan Blue
Wounds and Injuries
Crystallization
NADP
Oxidants
Hydrogen Peroxide
Lipid Peroxidation
Glutathione
Cell Survival
Epithelial Cells

Keywords

  • Calcium oxalate
  • Citric acid
  • Epithelium
  • Kidney
  • Reactive oxygen species

ASJC Scopus subject areas

  • Urology

Cite this

Citrate provides protection against oxalate and calcium oxalate crystal induced oxidative damage to renal epithelium. / Byer, Karen; Khan, Saeed R.

In: Journal of Urology, Vol. 173, No. 2, 02.2005, p. 640-646.

Research output: Contribution to journalArticle

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AB - Purpose: Oxalate and calcium oxalate (CaOx) crystals are injurious to renal epithelial cells. The injury is caused by the production of reactive oxygen species (ROS). Citrate is a well-known inhibitor of CaOx crystallization and as such it is one of the major therapeutic agents prescribed. Since citrate increases cellular reduced nicotinamide adenine dinucleotide phosphate and glutathione (GSH), we hypothesized that exogenously administered citrate should act as an anti-oxidant and protect cells from oxalate induced injury. Materials and Methods: We exposed LLC-PK1 and MDCK cells to 500 νM/ml oxalate or 150 μg/cm2 calcium oxalate crystals for 30, 60 and 180 minutes with or without 3 mg/ml citrate in the medium. We determined cell viability by lactate dehydrogenase release and trypan blue exclusion, ROS involvement by changes in hydrogen peroxide and GSH, and lipid peroxidation by quantifying 8-isoprostane. Results: The presence of citrate was associated with significant decrease in lactate dehydrogenase release (p

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