Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro

Yoshinori Go, Shravan K. Chintala, Alan Rayford, Evangelin Gagercas, Francis Ali-Osman, Boyapati Venkaiah, Raymond Sawaya, Ziya Gokaslan, Garth L. Nicolson, J. S. Rao

Research output: Contribution to journalArticle

Abstract

Glioblastomas extensively invade the surrounding normal brain tissue, with a concomitant expression of various proteolytic enzymes, in particular urokinase-type plasminogen activator (uPA). In this study we used cisdiamminedichloroplatinum (cisplatin) and 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), commonly used anti-cancer drugs for the treatment of glioblastomas, to study the expression of uPA in three human glioblastoma cell lines in vitro. Cells were treated with 25 μM cisplatin and 50 μM BCNU, and uPA levels were estimated by fibrin zymography during a 72-h time course. Treatment of glioblastoma cells with cisplatin resulted in significantly decreased levels of uPA in serum-free conditioned medium and cell extracts, compared to BCNU-treated and untreated cell lines. Quantitative levels of uPA enzyme activity assessed by scanning laser densitometry and uPA protein by ELISA using antibody against uPA showed decreased levels of uPA in cisplatin-treated glioma cell lines relative to BCNU and untreated cell lines. Our results suggest that anti-tumor compound, cisplatin, may exert its anti-neoplastic effects by inhibiting uPA in malignant glioblastomas.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalClinical and Experimental Metastasis
Volume15
Issue number4
DOIs
StatePublished - Jul 22 1997

Keywords

  • BCNU
  • Cisplatin
  • Glioblastoma
  • Invasion
  • uPA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Go, Y., Chintala, S. K., Rayford, A., Gagercas, E., Ali-Osman, F., Venkaiah, B., Sawaya, R., Gokaslan, Z., Nicolson, G. L., & Rao, J. S. (1997). Cisplatin but not BCNU inhibits urokinase-type plasminogen activator levels in human glioblastoma cell lines in vitro. Clinical and Experimental Metastasis, 15(4), 447-452. https://doi.org/10.1023/A:1018462507706