TY - JOUR
T1 - Circulating T cell-monocyte complexes are markers of immune perturbations
AU - Burel, Julie G.
AU - Pomaznoy, Mikhail
AU - Arlehamn, Cecilia S.Lindestam
AU - Weiskopf, Daniela
AU - da Silva Antunes, Ricardo
AU - Jung, Yunmin
AU - Babor, Mariana
AU - Schulten, Veronique
AU - Seumois, Gregory
AU - Greenbaum, Jason A.
AU - Premawansa, Sunil
AU - Premawansa, Gayani
AU - Wijewickrama, Ananda
AU - Vidanagama, Dhammika
AU - Gunasena, Bandu
AU - Tippalagama, Rashmi
AU - de Silva, Aruna D.
AU - Gilman, Robert H.
AU - Saito, Mayuko
AU - Taplitz, Randy
AU - Ley, Klaus
AU - Vijayanand, Pandurangan
AU - Sette, Alessandro
AU - Peters, Bjoern
N1 - Publisher Copyright:
© Burel et al.
PY - 2019/6
Y1 - 2019/6
N2 - Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artifacts and thus ignored in data acquisition and analysis, are the result of biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be re-visited.
AB - Our results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artifacts and thus ignored in data acquisition and analysis, are the result of biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell-monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be re-visited.
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U2 - 10.7554/eLife.46045.001
DO - 10.7554/eLife.46045.001
M3 - Article
C2 - 31237234
AN - SCOPUS:85071891394
SN - 2050-084X
VL - 8
JO - eLife
JF - eLife
M1 - e46045
ER -