Circulating proviral HIV DNA and HIV-associated dementia

Bruce Shiramizu, Suzanne Gartner, Andrew Williams, Cecilia Shikuma, Silvia Ratto-Kim, Michael Watters, Joleen Aguon, Victor Valcour

Research output: Contribution to journalArticle

Abstract

Objective: Individuals continue to develop HIV-1-associated dementia (HAD) despite treatment with highly active antiretroviral therapy (HAART). Monocytes/macrophages (M/MΦ) can harbor proviral DNA that is not eradicated by HAART. To determine if HAD is associated with the level of HIV-1 infection within circulating leukocytes, we quantified HIV-1 DNA copy number in peripheral blood mononuclear cells (PBMC), and in PBMC subsets. Design: Cross-sectional analysis within the Hawaii Aging with HIV Cohort comparing participants with HAD to those with normal cognition (NC). Methods: Real-time PCR assays assessing HIV DNA copy number/1 × 106 cells were performed on PBMC and subsets. Results. Individuals with HAD (n = 27) had a median (interquartile range) of 9.11 (37.20) HIV DNA per 1 × 106 PBMC compared to 0.49 (0.89) HIV DNA per 1 × 106 PBMC in individuals with NC (n = 22). Using a univariate analysis in the subset of individuals with undetectable viral load (HAD, n = 11 ; NC, n = 13), the odds of HAD attributable to HIV DNA copy number was 2.76 (1.28-5.94), P <0.01. Preliminary analysis of a small subset of patients (n = 5) suggested that the primary source of HIV DNA may be the activated M/MΦ (CD14/CD16) subset. Conclusions. These findings suggest a potentially important association between circulating provirus and HAD.

Original languageEnglish (US)
Pages (from-to)45-52
Number of pages8
JournalAIDS
Volume19
Issue number1
StatePublished - Jan 3 2005

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AIDS Dementia Complex
HIV-1
HIV
Dementia
DNA
Blood Cells
Cognition
Highly Active Antiretroviral Therapy
Monocytes
Macrophages
Proviruses
Viral Load
HIV Infections
Real-Time Polymerase Chain Reaction
Leukocytes
Cross-Sectional Studies

Keywords

  • Dementia
  • DNA
  • HIV-1
  • Provirus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Shiramizu, B., Gartner, S., Williams, A., Shikuma, C., Ratto-Kim, S., Watters, M., ... Valcour, V. (2005). Circulating proviral HIV DNA and HIV-associated dementia. AIDS, 19(1), 45-52.

Circulating proviral HIV DNA and HIV-associated dementia. / Shiramizu, Bruce; Gartner, Suzanne; Williams, Andrew; Shikuma, Cecilia; Ratto-Kim, Silvia; Watters, Michael; Aguon, Joleen; Valcour, Victor.

In: AIDS, Vol. 19, No. 1, 03.01.2005, p. 45-52.

Research output: Contribution to journalArticle

Shiramizu, B, Gartner, S, Williams, A, Shikuma, C, Ratto-Kim, S, Watters, M, Aguon, J & Valcour, V 2005, 'Circulating proviral HIV DNA and HIV-associated dementia', AIDS, vol. 19, no. 1, pp. 45-52.
Shiramizu B, Gartner S, Williams A, Shikuma C, Ratto-Kim S, Watters M et al. Circulating proviral HIV DNA and HIV-associated dementia. AIDS. 2005 Jan 3;19(1):45-52.
Shiramizu, Bruce ; Gartner, Suzanne ; Williams, Andrew ; Shikuma, Cecilia ; Ratto-Kim, Silvia ; Watters, Michael ; Aguon, Joleen ; Valcour, Victor. / Circulating proviral HIV DNA and HIV-associated dementia. In: AIDS. 2005 ; Vol. 19, No. 1. pp. 45-52.
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