Abstract
Objective: Individuals continue to develop HIV-1-associated dementia (HAD) despite treatment with highly active antiretroviral therapy (HAART). Monocytes/macrophages (M/MΦ) can harbor proviral DNA that is not eradicated by HAART. To determine if HAD is associated with the level of HIV-1 infection within circulating leukocytes, we quantified HIV-1 DNA copy number in peripheral blood mononuclear cells (PBMC), and in PBMC subsets. Design: Cross-sectional analysis within the Hawaii Aging with HIV Cohort comparing participants with HAD to those with normal cognition (NC). Methods: Real-time PCR assays assessing HIV DNA copy number/1 × 106 cells were performed on PBMC and subsets. Results. Individuals with HAD (n = 27) had a median (interquartile range) of 9.11 (37.20) HIV DNA per 1 × 106 PBMC compared to 0.49 (0.89) HIV DNA per 1 × 106 PBMC in individuals with NC (n = 22). Using a univariate analysis in the subset of individuals with undetectable viral load (HAD, n = 11 ; NC, n = 13), the odds of HAD attributable to HIV DNA copy number was 2.76 (1.28-5.94), P <0.01. Preliminary analysis of a small subset of patients (n = 5) suggested that the primary source of HIV DNA may be the activated M/MΦ (CD14/CD16) subset. Conclusions. These findings suggest a potentially important association between circulating provirus and HAD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-52 |
| Number of pages | 8 |
| Journal | AIDS |
| Volume | 19 |
| Issue number | 1 |
| State | Published - Jan 3 2005 |
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Keywords
- Dementia
- DNA
- HIV-1
- Provirus
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Circulating proviral HIV DNA and HIV-associated dementia. / Shiramizu, Bruce; Gartner, Suzanne; Williams, Andrew; Shikuma, Cecilia; Ratto-Kim, Silvia; Watters, Michael; Aguon, Joleen; Valcour, Victor.
In: AIDS, Vol. 19, No. 1, 03.01.2005, p. 45-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Circulating proviral HIV DNA and HIV-associated dementia
AU - Shiramizu, Bruce
AU - Gartner, Suzanne
AU - Williams, Andrew
AU - Shikuma, Cecilia
AU - Ratto-Kim, Silvia
AU - Watters, Michael
AU - Aguon, Joleen
AU - Valcour, Victor
PY - 2005/1/3
Y1 - 2005/1/3
N2 - Objective: Individuals continue to develop HIV-1-associated dementia (HAD) despite treatment with highly active antiretroviral therapy (HAART). Monocytes/macrophages (M/MΦ) can harbor proviral DNA that is not eradicated by HAART. To determine if HAD is associated with the level of HIV-1 infection within circulating leukocytes, we quantified HIV-1 DNA copy number in peripheral blood mononuclear cells (PBMC), and in PBMC subsets. Design: Cross-sectional analysis within the Hawaii Aging with HIV Cohort comparing participants with HAD to those with normal cognition (NC). Methods: Real-time PCR assays assessing HIV DNA copy number/1 × 106 cells were performed on PBMC and subsets. Results. Individuals with HAD (n = 27) had a median (interquartile range) of 9.11 (37.20) HIV DNA per 1 × 106 PBMC compared to 0.49 (0.89) HIV DNA per 1 × 106 PBMC in individuals with NC (n = 22). Using a univariate analysis in the subset of individuals with undetectable viral load (HAD, n = 11 ; NC, n = 13), the odds of HAD attributable to HIV DNA copy number was 2.76 (1.28-5.94), P <0.01. Preliminary analysis of a small subset of patients (n = 5) suggested that the primary source of HIV DNA may be the activated M/MΦ (CD14/CD16) subset. Conclusions. These findings suggest a potentially important association between circulating provirus and HAD.
AB - Objective: Individuals continue to develop HIV-1-associated dementia (HAD) despite treatment with highly active antiretroviral therapy (HAART). Monocytes/macrophages (M/MΦ) can harbor proviral DNA that is not eradicated by HAART. To determine if HAD is associated with the level of HIV-1 infection within circulating leukocytes, we quantified HIV-1 DNA copy number in peripheral blood mononuclear cells (PBMC), and in PBMC subsets. Design: Cross-sectional analysis within the Hawaii Aging with HIV Cohort comparing participants with HAD to those with normal cognition (NC). Methods: Real-time PCR assays assessing HIV DNA copy number/1 × 106 cells were performed on PBMC and subsets. Results. Individuals with HAD (n = 27) had a median (interquartile range) of 9.11 (37.20) HIV DNA per 1 × 106 PBMC compared to 0.49 (0.89) HIV DNA per 1 × 106 PBMC in individuals with NC (n = 22). Using a univariate analysis in the subset of individuals with undetectable viral load (HAD, n = 11 ; NC, n = 13), the odds of HAD attributable to HIV DNA copy number was 2.76 (1.28-5.94), P <0.01. Preliminary analysis of a small subset of patients (n = 5) suggested that the primary source of HIV DNA may be the activated M/MΦ (CD14/CD16) subset. Conclusions. These findings suggest a potentially important association between circulating provirus and HAD.
KW - Dementia
KW - DNA
KW - HIV-1
KW - Provirus
UR - http://www.scopus.com/inward/record.url?scp=12144260890&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12144260890&partnerID=8YFLogxK
M3 - Article
C2 - 15627032
AN - SCOPUS:12144260890
VL - 19
SP - 45
EP - 52
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 1
ER -