TY - JOUR
T1 - Circulating HFMD-associated coxsackievirus A16 is genetically and phenotypically distinct from the prototype CV-A16
AU - Wei, Wei
AU - Guo, Haoran
AU - Li, Jingliang
AU - Ren, Sangsang
AU - Wei, Zhenhong
AU - Bao, Wanguo
AU - Hu, Xiaoming
AU - Zhao, Ke
AU - Zhang, Wenyan
AU - Zhou, Yulai
AU - Sun, Fei
AU - Markham, Richard
AU - Yu, Xiao Fang
N1 - Funding Information:
We thank Xin Li, Xianjun Liu, Xiaomei Wang and Xin Liu for technical assistance and Deborah McClellan and Julia Romano for editorial assistance. The authors also gratefully acknowledge scholarship support from the China Scholarship Council for Wei Wei.
PY - 2014/4/15
Y1 - 2014/4/15
N2 - Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes.
AB - Human enteroviruses (HEV) have been linked to hand, foot, and mouth disease (HFMD) in the Pacific and Southeast Asia for decades. Many cases of HFMD have been attributed to coxsackievirus A16 (CV-A16, CA16), based on only partial viral genome determination. Viral phenotypes are also poorly defined. Herein, we have genetically and phenotypically characterized multiple circulating CV-A16 viruses from HFMD patients and determined multiple full-length sequences of these circulating viruses. We discovered that the circulating CV-A16 viruses from HFMD patients are genetically distinct from the proto-type CV-A16 G10. We have also isolated circulating CV-A16 viruses from hospitalized HFMD patients and compared their virological differences. Interestingly, circulating CV-A16 viruses are more pathogenic in a neonatal mouse model than is CV-A16 G10. Thus, we have found circulating recombinant forms of CV-A16 (CRF CV-A16) that are related to, but different from, the prototype CV-A16 G10 that have distinct biological phenotypes.
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U2 - 10.1371/journal.pone.0094746
DO - 10.1371/journal.pone.0094746
M3 - Article
C2 - 24736564
AN - SCOPUS:84899732596
VL - 9
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 4
M1 - e94746
ER -