Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model

Akhil Maheshwari, Robert D. Christensen, Darlene A. Calhoun, Reed A. Dimmitt, Atilano Lacson

Research output: Contribution to journalArticle

Abstract

Background: CXC-chemokines bearing the glutamic acid-leucine-arginine (ELR) motif (ELR+ CXC chemokines) are potent neutrophil chemoattractants and hence may play a role in mucosal injury seen with intestinal ischemia-reperfusion (I/R). Methods: Serum concentrations of ELR+ CXC chemokines (keratinocyte-derived chemokine(KC)/CXC ligand (CXCL) 1, macrophage inflammatory protein (MIP)-2/CXCL 2/3, lipopolysaccharide-induced CXC chemokine (LIX)/CXCL5, and lungkine/CXCL15) were measured in a murine intestinal I/R model. Fifteen 4-week-old wild-type mice were studied in three subgroups: sham, ischemia (superior mesenteric artery [SMA] clamping for 60 min) and ischemia-reperfusion (SMA clamping for 60 min followed by reperfusion for 90 min). Results: Concentrations of KC/CXCL1 and MIP-2/CXCL2/3 in sham-treated animals (145 ± 123 and 107 ± 55 pg/mL, respectively) and the ischemia subgroup (646 ± 413 and 226 ± 129 pg/mL) were similar, but concentrations were significantly higher with reperfusion (6398 ± 2297, p<.001 and 874 ± 790 pg/mL, p = .04). LIX/CXCL5 and lungkine/CXCL15 concentrations did not change significantly with ischemia or following I/R. KC/CXCL1 and MIP-2/CXCL2/3 concentrations correlated positively with the severity of mucosal injury and with each other, whereas a negative relationship was observed between LIX/CXCL5 concentrations and microscopic injury scores. Conclusions: Development of mucosal injury in intestinal I/R is associated with increased serum concentrations of KC/CXCL1 and MIP-2/CXCL2/3, but not with those of LIX/CXCL5 and lungkine/CXCL15.

Original languageEnglish (US)
Pages (from-to)145-157
Number of pages13
JournalFetal and Pediatric Pathology
Volume23
Issue number2-3
DOIs
StatePublished - Mar 1 2004
Externally publishedYes

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CXC Chemokines
Reperfusion
Chemokine CXCL2
Ischemia
Chemokine CXCL1
Superior Mesenteric Artery
Wounds and Injuries
Constriction
Chemokine CXCL5
Ligands
Chemotactic Factors
Serum
Leucine
Lipopolysaccharides
Arginine
Glutamic Acid
Neutrophils
keratinocyte-derived chemokines

Keywords

  • Chemokines
  • Intestine
  • Ischemia
  • Murine
  • Reperfusion

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pathology and Forensic Medicine

Cite this

Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. / Maheshwari, Akhil; Christensen, Robert D.; Calhoun, Darlene A.; Dimmitt, Reed A.; Lacson, Atilano.

In: Fetal and Pediatric Pathology, Vol. 23, No. 2-3, 01.03.2004, p. 145-157.

Research output: Contribution to journalArticle

Maheshwari, Akhil ; Christensen, Robert D. ; Calhoun, Darlene A. ; Dimmitt, Reed A. ; Lacson, Atilano. / Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. In: Fetal and Pediatric Pathology. 2004 ; Vol. 23, No. 2-3. pp. 145-157.
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AU - Calhoun, Darlene A.

AU - Dimmitt, Reed A.

AU - Lacson, Atilano

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N2 - Background: CXC-chemokines bearing the glutamic acid-leucine-arginine (ELR) motif (ELR+ CXC chemokines) are potent neutrophil chemoattractants and hence may play a role in mucosal injury seen with intestinal ischemia-reperfusion (I/R). Methods: Serum concentrations of ELR+ CXC chemokines (keratinocyte-derived chemokine(KC)/CXC ligand (CXCL) 1, macrophage inflammatory protein (MIP)-2/CXCL 2/3, lipopolysaccharide-induced CXC chemokine (LIX)/CXCL5, and lungkine/CXCL15) were measured in a murine intestinal I/R model. Fifteen 4-week-old wild-type mice were studied in three subgroups: sham, ischemia (superior mesenteric artery [SMA] clamping for 60 min) and ischemia-reperfusion (SMA clamping for 60 min followed by reperfusion for 90 min). Results: Concentrations of KC/CXCL1 and MIP-2/CXCL2/3 in sham-treated animals (145 ± 123 and 107 ± 55 pg/mL, respectively) and the ischemia subgroup (646 ± 413 and 226 ± 129 pg/mL) were similar, but concentrations were significantly higher with reperfusion (6398 ± 2297, p<.001 and 874 ± 790 pg/mL, p = .04). LIX/CXCL5 and lungkine/CXCL15 concentrations did not change significantly with ischemia or following I/R. KC/CXCL1 and MIP-2/CXCL2/3 concentrations correlated positively with the severity of mucosal injury and with each other, whereas a negative relationship was observed between LIX/CXCL5 concentrations and microscopic injury scores. Conclusions: Development of mucosal injury in intestinal I/R is associated with increased serum concentrations of KC/CXCL1 and MIP-2/CXCL2/3, but not with those of LIX/CXCL5 and lungkine/CXCL15.

AB - Background: CXC-chemokines bearing the glutamic acid-leucine-arginine (ELR) motif (ELR+ CXC chemokines) are potent neutrophil chemoattractants and hence may play a role in mucosal injury seen with intestinal ischemia-reperfusion (I/R). Methods: Serum concentrations of ELR+ CXC chemokines (keratinocyte-derived chemokine(KC)/CXC ligand (CXCL) 1, macrophage inflammatory protein (MIP)-2/CXCL 2/3, lipopolysaccharide-induced CXC chemokine (LIX)/CXCL5, and lungkine/CXCL15) were measured in a murine intestinal I/R model. Fifteen 4-week-old wild-type mice were studied in three subgroups: sham, ischemia (superior mesenteric artery [SMA] clamping for 60 min) and ischemia-reperfusion (SMA clamping for 60 min followed by reperfusion for 90 min). Results: Concentrations of KC/CXCL1 and MIP-2/CXCL2/3 in sham-treated animals (145 ± 123 and 107 ± 55 pg/mL, respectively) and the ischemia subgroup (646 ± 413 and 226 ± 129 pg/mL) were similar, but concentrations were significantly higher with reperfusion (6398 ± 2297, p<.001 and 874 ± 790 pg/mL, p = .04). LIX/CXCL5 and lungkine/CXCL15 concentrations did not change significantly with ischemia or following I/R. KC/CXCL1 and MIP-2/CXCL2/3 concentrations correlated positively with the severity of mucosal injury and with each other, whereas a negative relationship was observed between LIX/CXCL5 concentrations and microscopic injury scores. Conclusions: Development of mucosal injury in intestinal I/R is associated with increased serum concentrations of KC/CXCL1 and MIP-2/CXCL2/3, but not with those of LIX/CXCL5 and lungkine/CXCL15.

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