Circulating clonotypic B cells in classic Hodgkin lymphoma

Richard J Jones, Christopher Gocke, Yvette L. Kasamon, Carole B. Miller, Brandy Perkins, James P. Barber, Milada S. Vala, Jonathan M. Gerber, Lan L. Gellert, Mark Siedner, Mario Victor Lemas, Sarah Brennan, Richard F Ambinder, William Matsui

Research output: Contribution to journalArticle

Abstract

Although Hodgkin and Reed-Sternberg (HRS) cells are B lymphoid cells, they are unlike any normal cells of that lineage. Moreover, the limited proliferative potential of HRS cells belies the clinical aggressiveness of Hodgkin lymphoma (HL). More than 20 years ago, the L428 HL cell line was reported to contain a small population of phenotypic B cells that appeared responsible for the continued generation of HRS cells. This observation, however, has never been corroborated, and such clonotypic B cells have never been documented in HL patients. We found that both the L428 and KM-H2 HL cell lines contained rare B-cell subpopulations responsible for the generation and maintenance of the predominant HRS cell population. The B cells within the HL cell lines expressed immunoglobulin light chain, the memory B-cell antigen CD27, and the stem cell marker aldehyde dehydrogenase (ALDH). Clonal CD27+ALDHhigh B cells, sharing immunoglobulin gene rearrangements with lymph node HRS cells, were also detected in the blood of most newly diagnosed HL patients regardless of stage. Although the clinical significance of circulating clonotypic B cells in HL remains unclear, these data suggest they may be the initiating cells for HL.

Original languageEnglish (US)
Pages (from-to)5920-5926
Number of pages7
JournalBlood
Volume113
Issue number23
DOIs
StatePublished - 2009

Fingerprint

Hodgkin Disease
B-Lymphocytes
Cells
Reed-Sternberg Cells
Cell Line
CD27 Antigens
Immunoglobulin Light Chains
Aldehyde Dehydrogenase
Immunoglobulin Genes
Stem cells
Gene Rearrangement
Cell Lineage
Immunoglobulins
Blood
Population
Genes
Stem Cells
Data storage equipment
Lymph Nodes
Maintenance

ASJC Scopus subject areas

  • Hematology
  • Biochemistry
  • Cell Biology
  • Immunology

Cite this

Jones, R. J., Gocke, C., Kasamon, Y. L., Miller, C. B., Perkins, B., Barber, J. P., ... Matsui, W. (2009). Circulating clonotypic B cells in classic Hodgkin lymphoma. Blood, 113(23), 5920-5926. https://doi.org/10.1182/blood-2008-11-189688

Circulating clonotypic B cells in classic Hodgkin lymphoma. / Jones, Richard J; Gocke, Christopher; Kasamon, Yvette L.; Miller, Carole B.; Perkins, Brandy; Barber, James P.; Vala, Milada S.; Gerber, Jonathan M.; Gellert, Lan L.; Siedner, Mark; Lemas, Mario Victor; Brennan, Sarah; Ambinder, Richard F; Matsui, William.

In: Blood, Vol. 113, No. 23, 2009, p. 5920-5926.

Research output: Contribution to journalArticle

Jones, RJ, Gocke, C, Kasamon, YL, Miller, CB, Perkins, B, Barber, JP, Vala, MS, Gerber, JM, Gellert, LL, Siedner, M, Lemas, MV, Brennan, S, Ambinder, RF & Matsui, W 2009, 'Circulating clonotypic B cells in classic Hodgkin lymphoma', Blood, vol. 113, no. 23, pp. 5920-5926. https://doi.org/10.1182/blood-2008-11-189688
Jones, Richard J ; Gocke, Christopher ; Kasamon, Yvette L. ; Miller, Carole B. ; Perkins, Brandy ; Barber, James P. ; Vala, Milada S. ; Gerber, Jonathan M. ; Gellert, Lan L. ; Siedner, Mark ; Lemas, Mario Victor ; Brennan, Sarah ; Ambinder, Richard F ; Matsui, William. / Circulating clonotypic B cells in classic Hodgkin lymphoma. In: Blood. 2009 ; Vol. 113, No. 23. pp. 5920-5926.
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