Ciliary neurotrophic factor induces preprotachykinin A gene expression in the rat carotid body

Patrice R. Akins, Guimei Wu, Estelle B. Gauda

Research output: Contribution to journalArticlepeer-review

Abstract

Substance P (SP), a translational product of preprotachykinin-A (PPT-A) mRNA plays an important role in hypoxic chemotransmission in the rat carotid body. Although hypoxic exposure has been associated with an increase in SP content in the carotid body, factors that cause induction, regulation and release of PPT-A and SP in the carotid body remain to be elucidated. The purpose of this study was to investigate whether ciliary neurotrophic factor (CNTF), a factor that has been shown to regulate neurotransmitter phenotype in tissue from neural crest origin, could induce PPT-A gene expression in the rat carotid body. We used in situ hybridization histochemistry with radioactive ribonucleotide probes to investigate the effect of CNTF on PPT-A gene induction in the carotid body. Exposure of the rat superior cervical ganglia and carotid body to increasing concentrations of CNTF in culture resulted in up-regulation and induction of PPT-A mRNA, respectively.

Original languageEnglish (US)
Pages (from-to)95-98
Number of pages4
JournalNeuroscience Letters
Volume298
Issue number2
DOIs
StatePublished - Feb 2 2001

Keywords

  • Carotid body
  • Ciliary Neurotrophic Factor
  • In situ hybridization
  • Preprotachykinin mRNA
  • Substance P
  • Superior cervical Ganglia
  • mRNA trafficking

ASJC Scopus subject areas

  • Neuroscience(all)

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