Ciliary neurotrophic factor induces preprotachykinin A gene expression in the rat carotid body

Patrice R. Akins, Guimei Wu, Estelle B Gauda

Research output: Contribution to journalArticle

Abstract

Substance P (SP), a translational product of preprotachykinin-A (PPT-A) mRNA plays an important role in hypoxic chemotransmission in the rat carotid body. Although hypoxic exposure has been associated with an increase in SP content in the carotid body, factors that cause induction, regulation and release of PPT-A and SP in the carotid body remain to be elucidated. The purpose of this study was to investigate whether ciliary neurotrophic factor (CNTF), a factor that has been shown to regulate neurotransmitter phenotype in tissue from neural crest origin, could induce PPT-A gene expression in the rat carotid body. We used in situ hybridization histochemistry with radioactive ribonucleotide probes to investigate the effect of CNTF on PPT-A gene induction in the carotid body. Exposure of the rat superior cervical ganglia and carotid body to increasing concentrations of CNTF in culture resulted in up-regulation and induction of PPT-A mRNA, respectively.

Original languageEnglish (US)
Pages (from-to)95-98
Number of pages4
JournalNeuroscience Letters
Volume298
Issue number2
DOIs
StatePublished - Feb 2 2001

Fingerprint

Ciliary Neurotrophic Factor
Carotid Body
Gene Expression
Substance P
Ribonucleotides
Superior Cervical Ganglion
Messenger RNA
Neural Crest
In Situ Hybridization
Neurotransmitter Agents
preprotachykinin
Up-Regulation
Phenotype
Genes

Keywords

  • Carotid body
  • Ciliary Neurotrophic Factor
  • In situ hybridization
  • mRNA trafficking
  • Preprotachykinin mRNA
  • Substance P
  • Superior cervical Ganglia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ciliary neurotrophic factor induces preprotachykinin A gene expression in the rat carotid body. / Akins, Patrice R.; Wu, Guimei; Gauda, Estelle B.

In: Neuroscience Letters, Vol. 298, No. 2, 02.02.2001, p. 95-98.

Research output: Contribution to journalArticle

@article{193e35d9d75644d8af75909d6eaadbd5,
title = "Ciliary neurotrophic factor induces preprotachykinin A gene expression in the rat carotid body",
abstract = "Substance P (SP), a translational product of preprotachykinin-A (PPT-A) mRNA plays an important role in hypoxic chemotransmission in the rat carotid body. Although hypoxic exposure has been associated with an increase in SP content in the carotid body, factors that cause induction, regulation and release of PPT-A and SP in the carotid body remain to be elucidated. The purpose of this study was to investigate whether ciliary neurotrophic factor (CNTF), a factor that has been shown to regulate neurotransmitter phenotype in tissue from neural crest origin, could induce PPT-A gene expression in the rat carotid body. We used in situ hybridization histochemistry with radioactive ribonucleotide probes to investigate the effect of CNTF on PPT-A gene induction in the carotid body. Exposure of the rat superior cervical ganglia and carotid body to increasing concentrations of CNTF in culture resulted in up-regulation and induction of PPT-A mRNA, respectively.",
keywords = "Carotid body, Ciliary Neurotrophic Factor, In situ hybridization, mRNA trafficking, Preprotachykinin mRNA, Substance P, Superior cervical Ganglia",
author = "Akins, {Patrice R.} and Guimei Wu and Gauda, {Estelle B}",
year = "2001",
month = "2",
day = "2",
doi = "10.1016/S0304-3940(00)01717-1",
language = "English (US)",
volume = "298",
pages = "95--98",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Ciliary neurotrophic factor induces preprotachykinin A gene expression in the rat carotid body

AU - Akins, Patrice R.

AU - Wu, Guimei

AU - Gauda, Estelle B

PY - 2001/2/2

Y1 - 2001/2/2

N2 - Substance P (SP), a translational product of preprotachykinin-A (PPT-A) mRNA plays an important role in hypoxic chemotransmission in the rat carotid body. Although hypoxic exposure has been associated with an increase in SP content in the carotid body, factors that cause induction, regulation and release of PPT-A and SP in the carotid body remain to be elucidated. The purpose of this study was to investigate whether ciliary neurotrophic factor (CNTF), a factor that has been shown to regulate neurotransmitter phenotype in tissue from neural crest origin, could induce PPT-A gene expression in the rat carotid body. We used in situ hybridization histochemistry with radioactive ribonucleotide probes to investigate the effect of CNTF on PPT-A gene induction in the carotid body. Exposure of the rat superior cervical ganglia and carotid body to increasing concentrations of CNTF in culture resulted in up-regulation and induction of PPT-A mRNA, respectively.

AB - Substance P (SP), a translational product of preprotachykinin-A (PPT-A) mRNA plays an important role in hypoxic chemotransmission in the rat carotid body. Although hypoxic exposure has been associated with an increase in SP content in the carotid body, factors that cause induction, regulation and release of PPT-A and SP in the carotid body remain to be elucidated. The purpose of this study was to investigate whether ciliary neurotrophic factor (CNTF), a factor that has been shown to regulate neurotransmitter phenotype in tissue from neural crest origin, could induce PPT-A gene expression in the rat carotid body. We used in situ hybridization histochemistry with radioactive ribonucleotide probes to investigate the effect of CNTF on PPT-A gene induction in the carotid body. Exposure of the rat superior cervical ganglia and carotid body to increasing concentrations of CNTF in culture resulted in up-regulation and induction of PPT-A mRNA, respectively.

KW - Carotid body

KW - Ciliary Neurotrophic Factor

KW - In situ hybridization

KW - mRNA trafficking

KW - Preprotachykinin mRNA

KW - Substance P

KW - Superior cervical Ganglia

UR - http://www.scopus.com/inward/record.url?scp=0035793487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035793487&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(00)01717-1

DO - 10.1016/S0304-3940(00)01717-1

M3 - Article

C2 - 11163286

AN - SCOPUS:0035793487

VL - 298

SP - 95

EP - 98

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 2

ER -