@article{5846deeebf7a4cedb057f49c44f888b5,
title = "Cigarette smoke induces mitochondrial metabolic reprogramming in lung cells",
abstract = "Cellular transformation owing to cigarette smoking is due to chronic exposure and not acute. However, systematic studies to understand the molecular alterations in lung cells due to cigarette smoke are lacking. To understand these molecular alterations induced by chronic cigarette smoke exposure, we carried out tandem mass tag (TMT) based temporal proteomic profiling of lung cells exposed to cigarette smoke for upto 12 months. We identified 2620 proteins in total, of which 671 proteins were differentially expressed (1.5-fold) after 12 months of exposure. Prolonged exposure of lung cells to smoke for 12 months revealed dysregulation of oxidative phosphorylation and overexpression of enzymes involved in TCA cycle. In addition, we also observed overexpression of enzymes involved in glutamine metabolism, fatty acid degradation and lactate synthesis. This could possibly explain the availability of alternative source of carbon to TCA cycle apart from glycolytic pyruvate. Our data indicates that chronic exposure to cigarette smoke induces mitochondrial metabolic reprogramming in cells to support growth and survival.",
keywords = "Cigarette smoking, Mass spectrometry, Metabolism, NSCLC",
author = "Solanki, {Hitendra S.} and Niraj Babu and Jain, {Ankit P.} and Bhat, {Mohd Younis} and Puttamallesh, {Vinuth N.} and Jayshree Advani and Remya Raja and Mangalaparthi, {Kiran K.} and Kumar, {Mahesh M.} and Prasad, {T. S.Keshava} and Mathur, {Premendu Prakash} and David Sidransky and Harsha Gowda and Aditi Chatterjee",
note = "Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics (IOB), Bangalore. We thank the “Infosys Foundation” for research support to IOB. This work was supported by Department of Science and Technology (DST) grants (SERC/LS-439/2011 and SR/SO/HS/0208/2013). IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis (BT/01/COE/08/05). This work was supported by FAMRI-funded 072017_YCSA. RR is a recipient of Startup research scheme from DST. JA is recipient of Senior Research Fellowship and NB is recipient of Junior Research Fellowship from Council of Scientific and Industrial Research (CSIR). MYB and KKM are the recipients of Junior Research Fellowship from Department of Biotechnology (DBT), Government of India and University Grants Commission (UGC), Government of India, respectively. We thank Dr. S. K. Shankar and Dr. Anita Mahadevan (NIMHANS), for providing access to the microscopy imaging facility. Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics (IOB), Bangalore. We thank the “ Infosys Foundation ” for research support to IOB. This work was supported by Department of Science and Technology (DST) grants ( SERC/LS-439/2011 and SR/SO/HS/0208/2013 ). IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis ( BT/01/COE/08/05 ). This work was supported by FAMRI -funded 072017_YCSA . RR is a recipient of Startup research scheme from DST. JA is recipient of Senior Research Fellowship and NB is recipient of Junior Research Fellowship from Council of Scientific and Industrial Research (CSIR). MYB and KKM are the recipients of Junior Research Fellowship from Department of Biotechnology (DBT), Government of India and University Grants Commission (UGC), Government of India, respectively. We thank Dr. S. K. Shankar and Dr. Anita Mahadevan (NIMHANS), for providing access to the microscopy imaging facility. Publisher Copyright: {\textcopyright} 2017 Elsevier B.V. and Mitochondria Research Society",
year = "2018",
month = may,
doi = "10.1016/j.mito.2017.10.002",
language = "English (US)",
volume = "40",
pages = "58--70",
journal = "Mitochondrion",
issn = "1567-7249",
publisher = "Elsevier",
}