@article{6a126152646a40d6b2ccedbb59fd5a0f,
title = "Cigarette smoke and chewing tobacco alter expression of different sets of miRNAs in oral keratinocytes",
abstract = "Carcinogenic effect of tobacco in oral cancer is through chewing and/or smoking. Significant differences exist in development of oral cancer between tobacco users and non-users. However, molecular alterations induced by different forms of tobacco are yet to be fully elucidated. We developed cellular models of chronic exposure to chewing tobacco and cigarette smoke using immortalized oral keratinocytes. Chronic exposure to tobacco resulted in increased cell scattering and invasiveness in immortalized oral keratinocytes. miRNA sequencing using Illumina HiSeq 2500 resulted in the identification of 10 significantly dysregulated miRNAs (4 fold; p ≤ 0.05) in chewing tobacco treated cells and 6 in cigarette smoke exposed cells. We integrated this data with global proteomic data and identified 36 protein targets that showed inverse expression pattern in chewing tobacco treated cells and 16 protein targets that showed inverse expression in smoke exposed cells. In addition, we identified 6 novel miRNAs in chewing tobacco treated cells and 18 novel miRNAs in smoke exposed cells. Integrative analysis of dysregulated miRNAs and their targets indicates that signaling mechanisms leading to oncogenic transformation are distinct between both forms of tobacco. Our study demonstrates alterations in miRNA expression in oral cells in response to two frequently used forms of tobacco.",
author = "Bhat, {Mohd Younis} and Jayshree Advani and Pavithra Rajagopalan and Krishna Patel and Vishalakshi Nanjappa and Solanki, {Hitendra S.} and Patil, {Arun H.} and Bhat, {Firdous A.} and Mathur, {Premendu P.} and Bipin Nair and Prasad, {T. S.Keshava} and Califano, {Joseph A.} and David Sidransky and Harsha Gowda and Aditi Chatterjee",
note = "Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics (IOB), Bangalore. This work was supported by Department of Science and Technology (DST) grants (SERC/LS-439/2011 and SR/SO/HS/0208/2012) and FAMRI-funded 072017_YCSA. IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis (BT/01/COE/08/05). MYB is a recipient of Junior Research Fellowship from DBT. JA and PR are recipients of Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Government of India. Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics (IOB), Bangalore. This work was supported by Department of Science and Technology (DST) grants (SERC/LS-439/2011 and SR/SO/HS/0208/2012) and FAMRI-funded 072017-YCSA. IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis (BT/01/COE/08/05). MYB is a recipient of Junior Research Fellowship from DBT. JA and PR are recipients of Senior Research Fellowship from Council of Scientific and Industrial Research (CSIR), Government of India. Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41598-018-25498-2",
language = "English (US)",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}