CHX10 targets a subset of photoreceptor genes

Kimberley M. Dorval, Brian P. Bobechko, Hiroki Fujieda, Shiming Chen, Donald J Zack, Rod Bremner

Research output: Contribution to journalArticle

Abstract

The homeobox gene CHX10 is required for retinal progenitor cell proliferation early in retinogenesis and subsequently for bipolar neuron differentiation. To clarify the molecular mechanisms employed by CHX10 we sought to identify its target genes. In a yeast one-hybrid assay Chx10 interacted with the Ret1 site of the photoreceptor-specific gene Rhodopsin. Gel shift assays using in vitro translated protein confirmed that CHX10 binds to Ret1, but not to the similar Rhodopsin sites Ret4 and BAT-1. Using retinal nuclear lysates, we observed interactions between Chx10 and additional photoreceptor-specific elements including the PCE-1 (Rod arrestin/S-antigen) and the Cone opsin locus control region (Red/green cone opsin). However, chromatin immunoprecipitation assays revealed that in vivo, Chx10 bound sites upstream of the Rod arrestin and Interphotoreceptor retinoid-binding protein genes but not Rhodopsin or Cone opsin. Thus, in a chromatin context, Chx10 associates with a specific subset of elements that it binds with comparable apparent affinity in vitro. Our data suggest that CHX10 may target these motifs to inhibit rod photoreceptor gene expression in bipolar cells.

Original languageEnglish (US)
Pages (from-to)744-751
Number of pages8
JournalJournal of Biological Chemistry
Volume281
Issue number2
DOIs
StatePublished - Jan 13 2006

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Cone Opsins
Rhodopsin
Arrestin
Genes
Assays
Locus Control Region
Chromatin
Retinal Rod Photoreceptor Cells
Homeobox Genes
Chromatin Immunoprecipitation
Cell proliferation
Stem Cells
Yeasts
Gels
Cell Proliferation
Gene expression
Yeast
Neurons
Gene Expression
Antigens

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dorval, K. M., Bobechko, B. P., Fujieda, H., Chen, S., Zack, D. J., & Bremner, R. (2006). CHX10 targets a subset of photoreceptor genes. Journal of Biological Chemistry, 281(2), 744-751. https://doi.org/10.1074/jbc.M509470200

CHX10 targets a subset of photoreceptor genes. / Dorval, Kimberley M.; Bobechko, Brian P.; Fujieda, Hiroki; Chen, Shiming; Zack, Donald J; Bremner, Rod.

In: Journal of Biological Chemistry, Vol. 281, No. 2, 13.01.2006, p. 744-751.

Research output: Contribution to journalArticle

Dorval, KM, Bobechko, BP, Fujieda, H, Chen, S, Zack, DJ & Bremner, R 2006, 'CHX10 targets a subset of photoreceptor genes', Journal of Biological Chemistry, vol. 281, no. 2, pp. 744-751. https://doi.org/10.1074/jbc.M509470200
Dorval KM, Bobechko BP, Fujieda H, Chen S, Zack DJ, Bremner R. CHX10 targets a subset of photoreceptor genes. Journal of Biological Chemistry. 2006 Jan 13;281(2):744-751. https://doi.org/10.1074/jbc.M509470200
Dorval, Kimberley M. ; Bobechko, Brian P. ; Fujieda, Hiroki ; Chen, Shiming ; Zack, Donald J ; Bremner, Rod. / CHX10 targets a subset of photoreceptor genes. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 2. pp. 744-751.
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