@article{4e802740cb354b448faf51ee091b2d8e,
title = "Chronic shisha exposure alters phosphoproteome of oral keratinocytes",
abstract = "Shisha smoking has been epidemiologically linked to oral cancer. However, few studies have investigated the pathobiology of shisha-induced cellular transformation. We studied the effects of chronic shisha exposure (8 months) in an in vitro model using immortalized, non-neoplastic oral keratinocytes (OKF6/TERT1). Quantitative proteomic and phosphoproteomic analyses were performed on OKF6/TERT1 cells treated with shisha extract for a period of 8 months. Pathway analysis was carried out to identify significantly enriched biological processes in shisha-treated cells. Chronic shisha exposure resulted in increased cell scattering phenomenon in OKF6/TERT1 cells. Data analysis revealed differential phosphorylation of 164 peptides (fold change ≥1.5, p ≤ 0.0.5) corresponding to 136 proteins. Proteins associated with mTORC1 and EIF4F complexes involved in initiating protein translation were seen to be enriched upon shisha treatment. Network analysis also highlighted downregulation of proteins involved in Type I interferon signaling in shisha-treated cells. Quantitative phosphoproteomic approach elucidated global perturbations to the molecular milieu of oral keratinocytes upon shisha exposure. Further studies are needed to validate putative targets in oral cancer patients with shisha smoking history.",
keywords = "High-throughput, Hookah, Narghile, Orbitrap fusion, Waterpipe",
author = "Shankargouda Patil and Pavithra Rajagopalan and Krishna Patel and Tejaswini Subbannayya and Niraj Babu and Mohan, {Sonali V.} and Jayshree Advani and Gajanan Sathe and Shilpa Bhandi and Solanki, {Hitendra S.} and David Sidransky and Aditi Chatterjee and Harsha Gowda and Marco Ferrari",
note = "Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics. IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis (BT/01/COE/08/05). Krishna Patel and Niraj Babu are recipients of Senior Research Fellowships from the Council for Scientific and Industrial Research (CSIR), Government of India. We thank Dr. Anita Mahadevan of National Institute of Mental Health and Neurosciences (NIMHANS) for providing use of microscope facility. Funding Information: We thank the Department of Biotechnology (DBT), Government of India for research support to the Institute of Bioinformatics. IOB is supported by DBT Program Support on Neuroproteomics and infrastructure for proteomic data analysis (BT/01/COE/08/05). Krishna Patel and Niraj Babu are recipients of Senior Research Fellowships from the Council for Scientific and Industrial Research (CSIR), Government of India. We thank Dr. Anita Mahadevan of National Institute of Mental Health and Neurosciences (NIMHANS) for providing use of microscope facility. Publisher Copyright: {\textcopyright} 2019, The International CCN Society.",
year = "2019",
month = sep,
day = "1",
doi = "10.1007/s12079-019-00528-4",
language = "English (US)",
volume = "13",
pages = "281--289",
journal = "Journal of Cell Communication and Signaling",
issn = "1873-9601",
publisher = "Springer Netherlands",
number = "3",
}