Chronic restraint stress modulates expression of genes in murine spleen

Deling Yin, Ying Zhang, Charles Stuart, Junying Miao, Yi Zhang, Chuanfu Li, Xiao Zeng, Gregory Hanley, Jonathan Moorman, Zhiqiang Yao, Michael Woodruff

Research output: Contribution to journalArticle

Abstract

Psychological and physical stress can alter the immune system in both humans and animals. We have reported that mice subjected to chronic 12-h daily physical restraint for 2 days showed dramatic apoptosis in splenocytes. To identify genes that contribute to the splenocyte apoptosis, we compare gene expression in the spleens of restrained and unstressed mice using oligo microarrays consisting of 226 genes. We report here that mice subjected to chronic 12-h daily physical restraint for 2 days exhibited significantly altered expression of 50 of 226 genes. These genes included pro-apoptotic genes. We selected 5 genes of interest and confirmed the microarray results by real-time PCR. In this study, we identify a potentially important component of pro-apoptotic activity in restraint stress and suggest a possible target for anti-apoptotic therapy to protect splenocytes against stress-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)11-17
Number of pages7
JournalJournal of Neuroimmunology
Volume177
Issue number1-2
DOIs
StatePublished - Aug 1 2006

Keywords

  • Apoptosis
  • Gene expression
  • Mice
  • Microarray
  • Restraint stress

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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  • Cite this

    Yin, D., Zhang, Y., Stuart, C., Miao, J., Zhang, Y., Li, C., Zeng, X., Hanley, G., Moorman, J., Yao, Z., & Woodruff, M. (2006). Chronic restraint stress modulates expression of genes in murine spleen. Journal of Neuroimmunology, 177(1-2), 11-17. https://doi.org/10.1016/j.jneuroim.2006.05.004