Chronic resistance training decreases MuRF-1 and Atrogin-1 gene expression but does not modify Akt, GSK-3β and p70S6K levels in rats

Nelo Eidy Zanchi, Mário Alves de Siqueira Filho, Fabio Santos Lira, José Cesar Rosa, Alex Shimura Yamashita, Carla Roberta de Oliveira Carvalho, Marilia Seelaender, Antonio Herbert Lancha

Research output: Contribution to journalArticlepeer-review

Abstract

Long-term adaptation to resistance training is probably due to the cumulative molecular effects of each exercise session. Therefore, we studied in female Wistar rats the molecular effects of a chronic resistance training regimen (3 months) leading to skeletal muscle hypertrophy in the plantaris muscle. Our results demonstrated that muscle proteolytic genes MuRF-1 and Atrogin-1 were significantly decreased in the exercised group measured 24 h after the last resistance exercise session (41.64 and 61.19%, respectively; P < 0.05). Nonetheless, when measured at the same time point, 4EBP-1, GSK-3β and eIF2Bε mRNA levels and Akt, GSK-3β and p70S6K protein levels (regulators of translation initiation) were not modified. Such data suggests that if gene transcription constitutes a control point in the protein synthesis pathway this regulation probably occurs in early adaptation periods or during extreme situations leading to skeletal muscle remodeling. However, proteolytic gene expression is modified even after a prolonged resistance training regimen leading to moderate skeletal muscle hypertrophy.

Original languageEnglish (US)
Pages (from-to)415-423
Number of pages9
JournalEuropean Journal of Applied Physiology
Volume106
Issue number3
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Hypertrophy
  • Protein synthesis
  • Proteolysis
  • Skeletal muscle

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

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