Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels

Ellen M. Unterwald, Joshua M. Rubenfeld, Yasuo Imai, Jia Bei Wang, George R. Uhl, Mary Jeanne Kreek

Research output: Contribution to journalArticle

Abstract

Chronic administration of opioid antagonists has been shown to increase radioligand binding to brain opioid receptors. The present study was conducted to determine whether chronic exposure to the opioid antagonist naltrexone would similarly increase μ opioid receptor gene expression as measured by mRNA levels. Male Sprague-Dawley rats were administered naltrexone, 7-8 mg/kg/day, or saline by osmotic minipumps for 7 days. As expected, the density of μ opioid receptor binding sites was significantly higher in the brains of animals treated chronically with naltrexone as compared with saline treated control animals. However, μ opioid receptor mRNA content determined by a solution hybridization RNase protection assay was not significantly altered in any brain region investigated. These results indicate that the upregulation of μ opioid receptors as measured by radioligand binding is not accompanied by increased levels of μ receptor mRNA.

Original languageEnglish (US)
Pages (from-to)351-355
Number of pages5
JournalMolecular Brain Research
Volume33
Issue number2
DOIs
StatePublished - 1995

Fingerprint

Narcotic Antagonists
mu Opioid Receptor
Opioid Receptors
Up-Regulation
Naltrexone
Messenger RNA
Brain
Ribonucleases
Sprague Dawley Rats
Binding Sites
Gene Expression

Keywords

  • Mu opioid receptor
  • Mu opioid receptor mRNA
  • Naltrexone
  • Opioid receptor antagonist
  • Rat brain
  • Receptor regulation
  • Solution hybridization protection assay

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels. / Unterwald, Ellen M.; Rubenfeld, Joshua M.; Imai, Yasuo; Wang, Jia Bei; Uhl, George R.; Kreek, Mary Jeanne.

In: Molecular Brain Research, Vol. 33, No. 2, 1995, p. 351-355.

Research output: Contribution to journalArticle

Unterwald, Ellen M. ; Rubenfeld, Joshua M. ; Imai, Yasuo ; Wang, Jia Bei ; Uhl, George R. ; Kreek, Mary Jeanne. / Chronic opioid antagonist administration upregulates mu opioid receptor binding without altering mu opioid receptor mRNA levels. In: Molecular Brain Research. 1995 ; Vol. 33, No. 2. pp. 351-355.
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