Chronic inhibition of superoxide dismutase produces apoptotic death of spinal neurons

Jeffrey D Rothstein, Lynn A. Bristol, Betsy Hosler, Robert H. Brown, Ralph W. Kuncl

Research output: Contribution to journalArticle

Abstract

Mutations in the gene for Cu/Zn superoxide dismutase (SOD1) have been detected in some families with an autosomal dominant form of amyotrophic lateral sclerosis; these mutations appear to reduce the activity of this enzyme. To determine whether decreased SOD activity could contribute to motor neuron loss, SOD1 was inhibited chronically with either antisense oligodeoxynucleotides or diethyldithiocarbamate in spinal cord organotypic cultures. Chronic inhibition of SOD resulted in the apoptotic degeneration of spinal neurons, including motor neurons, over several weeks. Motor neuron loss was markedly potentiated by the inhibition of glutamate transport. In this paradigm, motor neuron toxicity could be entirely prevented by the antioxidant N-acetylcysteine and, to a lesser extent, by the non-N-methyl-D- aspartate glutamate receptor antagonist 1-(4-aminophenyl)-4-methyl-7,8- methylenedioxy-5H-2,3-benzodiazepine hydrochloride. These data support the hypothesis that the loss of motor neurons in familial amyotrophic lateral sclerosis could be due to a reduction in SOD1 activity, possibly potentiated by inefficient glutamate transport.

Original languageEnglish (US)
Pages (from-to)4155-4159
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number10
Publication statusPublished - May 10 1994

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Keywords

  • amyotrophic lateral sclerosis
  • antioxidant
  • free radicals
  • motor neuron
  • organotypic culture

ASJC Scopus subject areas

  • Genetics
  • General

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