Chronic compression of the dorsal root ganglion enhances mechanically evoked pain behavior and the activity of cutaneous nociceptors in mice

Tao Wang, Olivia Hurwitz, Steven G. Shimada, Lintao Qu, Kai Fu, Pu Zhang, Chao Ma, Robert H. LaMotte

Research output: Contribution to journalArticle

Abstract

Radicular pain in humans is usually caused by intraforaminal stenosis and other diseases affecting the spinal nerve, root, or dorsal root ganglion (DRG). Previous studies discovered that a chronic compression of the DRG (CCD) induced mechanical allodynia in rats and mice, with enhanced excitability of DRG neurons. We investigated whether CCD altered the pain-like behavior and also the responses of cutaneous nociceptors with unmyelinated axons (C-fibers) to a normally aversive punctate mechanical stimulus delivered to the hairy skin of the hind limb of the mouse. The incidence of a foot shaking evoked by indentation of the dorsum of foot with an aversive von Frey filament (tip diameter 200 μm, bending force 20 mN) was significantly higher in the foot ipsilateral to the CCD surgery as compared to the contralateral side on post-operative days 2 to 8. Mechanically-evoked action potentials were electrophysiologically recorded from the L3 DRG, in vivo, from cell bodies visually identified as expressing a transgenically labeled fluorescent marker (neurons expressing either the receptor MrgprA3 or MrgprD). After CCD, 26.7%of MrgprA3+ and 32.1% MrgprD+ neurons exhibited spontaneous activity (SA), while none of the unoperated control neurons had SA. MrgprA3+ and MrgprD+ neurons in the compressed DRG exhibited, in comparison with neurons from unoperated control mice, an increased response to the punctate mechanical stimuli for each force applied (6, 20, 40, and 80 mN). We conclude that CCD produced both a behavioral hyperalgesia and an enhanced response of cutaneous C-nociceptors to aversive punctate mechanical stimuli.

Original languageEnglish (US)
Article numbere0137512
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 10 2015

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Nociceptors
Spinal Ganglia
Neurons
pain
neurons
Diagnosis-Related Groups
Pain
Skin
mice
Foot
Hyperalgesia
spinal diseases
Spinal Nerve Roots
action potentials
limbs (animal)
Indentation
Evoked Potentials
skin (animal)
axons
Surgery

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chronic compression of the dorsal root ganglion enhances mechanically evoked pain behavior and the activity of cutaneous nociceptors in mice. / Wang, Tao; Hurwitz, Olivia; Shimada, Steven G.; Qu, Lintao; Fu, Kai; Zhang, Pu; Ma, Chao; LaMotte, Robert H.

In: PLoS One, Vol. 10, No. 9, e0137512, 10.09.2015.

Research output: Contribution to journalArticle

Wang, Tao ; Hurwitz, Olivia ; Shimada, Steven G. ; Qu, Lintao ; Fu, Kai ; Zhang, Pu ; Ma, Chao ; LaMotte, Robert H. / Chronic compression of the dorsal root ganglion enhances mechanically evoked pain behavior and the activity of cutaneous nociceptors in mice. In: PLoS One. 2015 ; Vol. 10, No. 9.
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