TY - JOUR
T1 - Chromosome arm-specific multicolor FISH
AU - Karhu, Ritva
AU - Ahlstedt-Soini, Minna
AU - Bittner, Michael
AU - Meltzer, Paul
AU - Trent, Jeffrey M.
AU - Isola, Jorma J.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - Several systems for 24-color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm-specific painting probes (arm-kit, comprising either p- or q-arms of all human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The mFISH-probe cocktail and the arm-kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm-kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers. (C) 2001 Wiley-Liss, Inc.
AB - Several systems for 24-color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm-specific painting probes (arm-kit, comprising either p- or q-arms of all human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The mFISH-probe cocktail and the arm-kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm-kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers. (C) 2001 Wiley-Liss, Inc.
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U2 - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1068>3.0.CO;2-9
DO - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1068>3.0.CO;2-9
M3 - Article
C2 - 11107184
AN - SCOPUS:0035213357
SN - 1045-2257
VL - 30
SP - 105
EP - 109
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 1
ER -