Chromosome arm-specific multicolor FISH

Ritva Karhu, Minna Ahlstedt-Soini, Michael Bittner, Paul Meltzer, Jeffrey M. Trent, Jorma J. Isola

Research output: Contribution to journalArticlepeer-review

Abstract

Several systems for 24-color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42-color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm-specific painting probes (arm-kit, comprising either p- or q-arms of all human chromosomes, except the p-arm of the acrocentric- and Y chromosomes). The mFISH-probe cocktail and the arm-kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm-kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers. (C) 2001 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalGenes Chromosomes and Cancer
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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