Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence

Ichabod Jung, Jay E. Reeder, Christopher Cox, Josephine F M Siddiqui, Mary J. O'Connell, Loretta Collins, Zhihong Yang, Edward M. Messing, Leon L. Wheeless

Research output: Contribution to journalArticle

Abstract

Purpose: Bladder irrigation specimens are effective for sampling the urothelium for detection of recurrent bladder cancer. These specimens can be evaluated by cytology or quantitative techniques. Proliferation and ploidy changes are readily detected using deoxyribonucleic acid (DNA) cytometry. Tumor associated chromosomal aberrations can be assayed using fluorescence in situ hybridization (FISH). The prognostic values of DNA cytometry, and chromosome 9 and 9p21 FISH on exfoliated cells from bladder irrigation specimens from 61 bladder cancer patients were evaluated. Materials and Methods: A total of 61 consecutive bladder irrigation specimens were obtained during cystoscopy. DNA cytometry was performed by image analysis. FISH was performed using a centromeric chromosome 9 probe and a cosmid contig (COSp16) probe to the CDKN2A/p16 tumor suppressor region of 9p21. Proportional hazards regression analysis was performed with statistical software to test the predictor variables of initial patient status (presence of tumor), COSp16 fraction (the proportion of COSp16 signals relative to centromeric probe signals), monosomic and hyperdisomic fractions of the chromosome 9 probe, and hyperdiploid fraction from DNA cytometry. Median time to recurrence was calculated using statistical software survival analysis. Results: Initial patient status and monosomy of chromosome 9 were predictive of bladder cancer recurrence (p

Original languageEnglish (US)
Pages (from-to)1900-1903
Number of pages4
JournalJournal of Urology
Volume162
Issue number6
StatePublished - Dec 1999
Externally publishedYes

Fingerprint

Monosomy
Chromosomes, Human, Pair 9
Fluorescence In Situ Hybridization
Urinary Bladder
Urinary Bladder Neoplasms
Recurrence
DNA
Neoplasms
Software
Urothelium
Cosmids
Cystoscopy
Polyploidy
Ploidies
Survival Analysis
Chromosome Aberrations
Cell Biology
Regression Analysis

Keywords

  • Bladder
  • Chromosomes, human
  • DNA
  • Fluorescence
  • Genetic markers
  • In situ hybridization
  • Pair 9

ASJC Scopus subject areas

  • Urology

Cite this

Jung, I., Reeder, J. E., Cox, C., Siddiqui, J. F. M., O'Connell, M. J., Collins, L., ... Wheeless, L. L. (1999). Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence. Journal of Urology, 162(6), 1900-1903.

Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence. / Jung, Ichabod; Reeder, Jay E.; Cox, Christopher; Siddiqui, Josephine F M; O'Connell, Mary J.; Collins, Loretta; Yang, Zhihong; Messing, Edward M.; Wheeless, Leon L.

In: Journal of Urology, Vol. 162, No. 6, 12.1999, p. 1900-1903.

Research output: Contribution to journalArticle

Jung, I, Reeder, JE, Cox, C, Siddiqui, JFM, O'Connell, MJ, Collins, L, Yang, Z, Messing, EM & Wheeless, LL 1999, 'Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence', Journal of Urology, vol. 162, no. 6, pp. 1900-1903.
Jung, Ichabod ; Reeder, Jay E. ; Cox, Christopher ; Siddiqui, Josephine F M ; O'Connell, Mary J. ; Collins, Loretta ; Yang, Zhihong ; Messing, Edward M. ; Wheeless, Leon L. / Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence. In: Journal of Urology. 1999 ; Vol. 162, No. 6. pp. 1900-1903.
@article{bc2fc8ad7b044ddbbab53d3c617c8b32,
title = "Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence",
abstract = "Purpose: Bladder irrigation specimens are effective for sampling the urothelium for detection of recurrent bladder cancer. These specimens can be evaluated by cytology or quantitative techniques. Proliferation and ploidy changes are readily detected using deoxyribonucleic acid (DNA) cytometry. Tumor associated chromosomal aberrations can be assayed using fluorescence in situ hybridization (FISH). The prognostic values of DNA cytometry, and chromosome 9 and 9p21 FISH on exfoliated cells from bladder irrigation specimens from 61 bladder cancer patients were evaluated. Materials and Methods: A total of 61 consecutive bladder irrigation specimens were obtained during cystoscopy. DNA cytometry was performed by image analysis. FISH was performed using a centromeric chromosome 9 probe and a cosmid contig (COSp16) probe to the CDKN2A/p16 tumor suppressor region of 9p21. Proportional hazards regression analysis was performed with statistical software to test the predictor variables of initial patient status (presence of tumor), COSp16 fraction (the proportion of COSp16 signals relative to centromeric probe signals), monosomic and hyperdisomic fractions of the chromosome 9 probe, and hyperdiploid fraction from DNA cytometry. Median time to recurrence was calculated using statistical software survival analysis. Results: Initial patient status and monosomy of chromosome 9 were predictive of bladder cancer recurrence (p",
keywords = "Bladder, Chromosomes, human, DNA, Fluorescence, Genetic markers, In situ hybridization, Pair 9",
author = "Ichabod Jung and Reeder, {Jay E.} and Christopher Cox and Siddiqui, {Josephine F M} and O'Connell, {Mary J.} and Loretta Collins and Zhihong Yang and Messing, {Edward M.} and Wheeless, {Leon L.}",
year = "1999",
month = "12",
language = "English (US)",
volume = "162",
pages = "1900--1903",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence

AU - Jung, Ichabod

AU - Reeder, Jay E.

AU - Cox, Christopher

AU - Siddiqui, Josephine F M

AU - O'Connell, Mary J.

AU - Collins, Loretta

AU - Yang, Zhihong

AU - Messing, Edward M.

AU - Wheeless, Leon L.

PY - 1999/12

Y1 - 1999/12

N2 - Purpose: Bladder irrigation specimens are effective for sampling the urothelium for detection of recurrent bladder cancer. These specimens can be evaluated by cytology or quantitative techniques. Proliferation and ploidy changes are readily detected using deoxyribonucleic acid (DNA) cytometry. Tumor associated chromosomal aberrations can be assayed using fluorescence in situ hybridization (FISH). The prognostic values of DNA cytometry, and chromosome 9 and 9p21 FISH on exfoliated cells from bladder irrigation specimens from 61 bladder cancer patients were evaluated. Materials and Methods: A total of 61 consecutive bladder irrigation specimens were obtained during cystoscopy. DNA cytometry was performed by image analysis. FISH was performed using a centromeric chromosome 9 probe and a cosmid contig (COSp16) probe to the CDKN2A/p16 tumor suppressor region of 9p21. Proportional hazards regression analysis was performed with statistical software to test the predictor variables of initial patient status (presence of tumor), COSp16 fraction (the proportion of COSp16 signals relative to centromeric probe signals), monosomic and hyperdisomic fractions of the chromosome 9 probe, and hyperdiploid fraction from DNA cytometry. Median time to recurrence was calculated using statistical software survival analysis. Results: Initial patient status and monosomy of chromosome 9 were predictive of bladder cancer recurrence (p

AB - Purpose: Bladder irrigation specimens are effective for sampling the urothelium for detection of recurrent bladder cancer. These specimens can be evaluated by cytology or quantitative techniques. Proliferation and ploidy changes are readily detected using deoxyribonucleic acid (DNA) cytometry. Tumor associated chromosomal aberrations can be assayed using fluorescence in situ hybridization (FISH). The prognostic values of DNA cytometry, and chromosome 9 and 9p21 FISH on exfoliated cells from bladder irrigation specimens from 61 bladder cancer patients were evaluated. Materials and Methods: A total of 61 consecutive bladder irrigation specimens were obtained during cystoscopy. DNA cytometry was performed by image analysis. FISH was performed using a centromeric chromosome 9 probe and a cosmid contig (COSp16) probe to the CDKN2A/p16 tumor suppressor region of 9p21. Proportional hazards regression analysis was performed with statistical software to test the predictor variables of initial patient status (presence of tumor), COSp16 fraction (the proportion of COSp16 signals relative to centromeric probe signals), monosomic and hyperdisomic fractions of the chromosome 9 probe, and hyperdiploid fraction from DNA cytometry. Median time to recurrence was calculated using statistical software survival analysis. Results: Initial patient status and monosomy of chromosome 9 were predictive of bladder cancer recurrence (p

KW - Bladder

KW - Chromosomes, human

KW - DNA

KW - Fluorescence

KW - Genetic markers

KW - In situ hybridization

KW - Pair 9

UR - http://www.scopus.com/inward/record.url?scp=0032728962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032728962&partnerID=8YFLogxK

M3 - Article

VL - 162

SP - 1900

EP - 1903

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 6

ER -