Chromosomal variations within aneuploid cancer lines

Takahiro Isaka, Andrea L. Nestor, Tadahiro Takada, David C. Allison

Research output: Contribution to journalArticlepeer-review

Abstract

Aneuploid cancers exhibit a wide spectrum of clinical aggressiveness, possibly because of varying chromosome compositions. To test this, karyotypes from the diploid CCD-34Lu fibroblast and the aneuploid A549 and SUIT-2 cancer lines underwent fluorescence in situ hybridization (FISH) and DAPI counterstaining. The number of DAPI-stained and FISH-identified chromosomes, 1-22, X,Y, as well as structural abnormalities, were counted and compared using the X2, Mann-Whitney rank sum test and the Levene's equality of variance. Virtually all of the evaluable diploid CCD-34Lu karyotypes had 46 chromosomes with two normal-appearing homologues. The aneuploid chromosome numbers per karyotype were highly variable, averaging 62 and 72 for the A549 and SUIT-2 lines, respectively. However, the A549 chromosome numbers were more narrowly distributed than the SUIT-2 karyotype chromosome numbers. Furthermore, 25% of the A549 chromosomes had structural abnormalities compared to only 7% of the SUIT-2 chromosomes. The chromosomal compositions of the aneuploid A549 and SUIT-2 cancer lines are widely divergent, suggesting that diverse genetic alterations, rather than chance, may govern the chromosome makeups of aneuploid cancers.

Original languageEnglish (US)
Pages (from-to)1343-1353
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number10
DOIs
StatePublished - Oct 1 2003

Keywords

  • Aneuploidy
  • Cell lines
  • Chromosomal abnormalities
  • Fluorescence in situ hybridization
  • Karyotypes

ASJC Scopus subject areas

  • Anatomy
  • Histology

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