Chromatography of methyl ethers of d-glucosamine

Y. C. Lee, J. Scocca

Research output: Contribution to journalArticle

Abstract

Identification of methyl ethers obtained by methylation and subsequent hydrolysis is a powerful technique for determination of linkage positions in structure studies of complex carbohydrates. Although methyl ethers of neutral sugars have been separated by various chromatographic methods, separation of methyl ethers of 2-amino-2-deoxy-d-glucose has been more difficult. Only recently, successful procedures for separation of methyl ethers of d-glucosamine based on thin-layer (1), gas (2), and column (3) chromatography have appeared in literature. We wish to report here a method for separation of d-glucosamine methyl ethers using a combination of partition and ion-exchange chromatography.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalAnalytical Biochemistry
Volume39
Issue number1
DOIs
StatePublished - 1971

Fingerprint

Methyl Ethers
Glucosamine
Chromatography
Methylation
Ion Exchange Chromatography
Sugars
Hydrolysis
Ion exchange
Gases
Carbohydrates
Glucose

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chromatography of methyl ethers of d-glucosamine. / Lee, Y. C.; Scocca, J.

In: Analytical Biochemistry, Vol. 39, No. 1, 1971, p. 24-28.

Research output: Contribution to journalArticle

Lee, Y. C. ; Scocca, J. / Chromatography of methyl ethers of d-glucosamine. In: Analytical Biochemistry. 1971 ; Vol. 39, No. 1. pp. 24-28.
@article{f82c2401fd5c4ae1b2ff551badd1f7e7,
title = "Chromatography of methyl ethers of d-glucosamine",
abstract = "Identification of methyl ethers obtained by methylation and subsequent hydrolysis is a powerful technique for determination of linkage positions in structure studies of complex carbohydrates. Although methyl ethers of neutral sugars have been separated by various chromatographic methods, separation of methyl ethers of 2-amino-2-deoxy-d-glucose has been more difficult. Only recently, successful procedures for separation of methyl ethers of d-glucosamine based on thin-layer (1), gas (2), and column (3) chromatography have appeared in literature. We wish to report here a method for separation of d-glucosamine methyl ethers using a combination of partition and ion-exchange chromatography.",
author = "Lee, {Y. C.} and J. Scocca",
year = "1971",
doi = "10.1016/0003-2697(71)90457-X",
language = "English (US)",
volume = "39",
pages = "24--28",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Chromatography of methyl ethers of d-glucosamine

AU - Lee, Y. C.

AU - Scocca, J.

PY - 1971

Y1 - 1971

N2 - Identification of methyl ethers obtained by methylation and subsequent hydrolysis is a powerful technique for determination of linkage positions in structure studies of complex carbohydrates. Although methyl ethers of neutral sugars have been separated by various chromatographic methods, separation of methyl ethers of 2-amino-2-deoxy-d-glucose has been more difficult. Only recently, successful procedures for separation of methyl ethers of d-glucosamine based on thin-layer (1), gas (2), and column (3) chromatography have appeared in literature. We wish to report here a method for separation of d-glucosamine methyl ethers using a combination of partition and ion-exchange chromatography.

AB - Identification of methyl ethers obtained by methylation and subsequent hydrolysis is a powerful technique for determination of linkage positions in structure studies of complex carbohydrates. Although methyl ethers of neutral sugars have been separated by various chromatographic methods, separation of methyl ethers of 2-amino-2-deoxy-d-glucose has been more difficult. Only recently, successful procedures for separation of methyl ethers of d-glucosamine based on thin-layer (1), gas (2), and column (3) chromatography have appeared in literature. We wish to report here a method for separation of d-glucosamine methyl ethers using a combination of partition and ion-exchange chromatography.

UR - http://www.scopus.com/inward/record.url?scp=0014984563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014984563&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(71)90457-X

DO - 10.1016/0003-2697(71)90457-X

M3 - Article

C2 - 5544600

AN - SCOPUS:0014984563

VL - 39

SP - 24

EP - 28

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -