Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Dec 23 1974|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology