Chromatographic separation of chromatin subunits

B. Ramsay Shaw, J. L. Corden, C. G. Sahasrabuddhe, K. E. Van Holde

Research output: Contribution to journalArticlepeer-review

Abstract

Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

Original languageEnglish (US)
Pages (from-to)1193-1198
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume61
Issue number4
DOIs
StatePublished - Dec 23 1974
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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