Chromatographic separation of chromatin subunits

B. Ramsay Shaw; J. L. Corden; C. G. Sahasrabuddhe; K. E. Van Holde

doi:10.1016/S0006-291X(74)80410-9

Chromatographic separation of chromatin subunits

B. Ramsay Shaw, J. L. Corden, C. G. Sahasrabuddhe, K. E. Van Holde

Research output: Contribution to journal › Article › peer-review

Abstract

Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

Original language	English (US)
Pages (from-to)	1193-1198
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	61
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(74)80410-9
State	Published - Dec 23 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).",

author = "Shaw, {B. Ramsay} and Corden, {J. L.} and Sahasrabuddhe, {C. G.} and {Van Holde}, {K. E.}",

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AU - Shaw, B. Ramsay

AU - Corden, J. L.

AU - Sahasrabuddhe, C. G.

AU - Van Holde, K. E.

PY - 1974/12/23

Y1 - 1974/12/23

N2 - Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

AB - Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

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