Chromatographic separation of chromatin subunits

B. Ramsay Shaw; J. L. Corden; C. G. Sahasrabuddhe; K. E. Van Holde

doi:10.1016/S0006-291X(74)80410-9

Chromatographic separation of chromatin subunits

B. Ramsay Shaw, J. L. Corden, C. G. Sahasrabuddhe, K. E. Van Holde

Research output: Contribution to journal › Article › peer-review

Abstract

Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

Original language	English (US)
Pages (from-to)	1193-1198
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	61
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(74)80410-9
State	Published - Dec 23 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(74)80410-9

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title = "Chromatographic separation of chromatin subunits",

abstract = "Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).",

author = "Shaw, {B. Ramsay} and Corden, {J. L.} and Sahasrabuddhe, {C. G.} and {Van Holde}, {K. E.}",

note = "Funding Information: ACKNOWLEDGEMENTS: We wish to thank Mr. Tim Kovacic for the PM2 Hae III fragments and Ms. Maureen Drury for excellent assistance in carrying out the ultracentrifuge measurements. The research on which this paper is based was supported by the National Science Foundation (Grant No. 37307). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1974",

month = dec,

day = "23",

doi = "10.1016/S0006-291X(74)80410-9",

language = "English (US)",

volume = "61",

pages = "1193--1198",

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T1 - Chromatographic separation of chromatin subunits

AU - Shaw, B. Ramsay

AU - Corden, J. L.

AU - Sahasrabuddhe, C. G.

AU - Van Holde, K. E.

N1 - Funding Information: ACKNOWLEDGEMENTS: We wish to thank Mr. Tim Kovacic for the PM2 Hae III fragments and Ms. Maureen Drury for excellent assistance in carrying out the ultracentrifuge measurements. The research on which this paper is based was supported by the National Science Foundation (Grant No. 37307). Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1974/12/23

Y1 - 1974/12/23

N2 - Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

AB - Calf thymus nuclei were partially digested with staphylococcal nuclease and the resulting chromatin particles separated by gel permeation chromatography. Individual column fractions were characterized by sedimentation velocity and circular dichroism. DNA isolated from these chromatin fractions was electrophoresed. The resulting data have enabled us to correlate the series of DNA molecules produced by in situ nuclease digestion (1) with nuclease resistant nucleoprotein particles from whole chromatin (2).

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DO - 10.1016/S0006-291X(74)80410-9

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

Chromatographic separation of chromatin subunits

Abstract

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