Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas

Cheng Ran Xu, Philip A. Cole, David J Meyers, Jay Kormish, Sharon Dent, Kenneth S. Zaret

Research output: Contribution to journalArticle

Abstract

Transcriptionally silent genes can be marked by histone modifications and regulatory proteins that indicate the genes' potential to be activated. Such marks have been identified in pluripotent cells, but it is unknown how such marks occur in descendant, multipotent embryonic cells that have restricted cell fate choices. We isolated mouse embryonic endoderm cells and assessed histone modifications at regulatory elements of silent genes that are activated upon liver or pancreas fate choices. We found that the liver and pancreas elements have distinct chromatin patterns. Furthermore, the histone acetyltransferase P300, recruited via bone morphogenetic protein signaling, and the histone methyltransferase Ezh2 have modulatory roles in the fate choice. These studies reveal a functional "prepattern" of chromatin states within multipotent progenitors and potential targets to modulate cell fate induction.

Original languageEnglish (US)
Pages (from-to)963-966
Number of pages4
JournalScience
Volume332
Issue number6032
DOIs
StatePublished - May 20 2011

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Histones
Chromatin
Pancreas
Histone Code
Liver
Histone Acetyltransferases
Endoderm
Bone Morphogenetic Proteins
Genes
Proteins

ASJC Scopus subject areas

  • General

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Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas. / Xu, Cheng Ran; Cole, Philip A.; Meyers, David J; Kormish, Jay; Dent, Sharon; Zaret, Kenneth S.

In: Science, Vol. 332, No. 6032, 20.05.2011, p. 963-966.

Research output: Contribution to journalArticle

Xu, CR, Cole, PA, Meyers, DJ, Kormish, J, Dent, S & Zaret, KS 2011, 'Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas', Science, vol. 332, no. 6032, pp. 963-966. https://doi.org/10.1126/science.1202845
Xu, Cheng Ran ; Cole, Philip A. ; Meyers, David J ; Kormish, Jay ; Dent, Sharon ; Zaret, Kenneth S. / Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas. In: Science. 2011 ; Vol. 332, No. 6032. pp. 963-966.
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