Chromatin loosening by poly(ADP)-ribose polymerase (PARP) at Drosophila puff loci

Alexei Tulin, Allan Spradling

Research output: Contribution to journalArticle

Abstract

Steroid response and stress-activated genes such as hsp7O undergo puffing in Drosophila larval salivary glands, a local loosening of polytene chromatin structure associated with gene induction. We find that puffs acquire elevated levels of adenosine diphosphate (ADP)-ribose modified proteins and that poly(ADP) ribose polymerase (PARP) is required to produce normal-sized puffs and normal amounts of Hsp7O after heat exposure. We propose that chromosomal PARP molecules become activated by developmental or environmental cues and strip nearby chromatin proteins off DNA to generate a puff. Such local loosening may facilitate transcription and may transiently make protein complexes more accessible to modification, promoting chromatin remodeling during development.

Original languageEnglish (US)
Pages (from-to)560-562
Number of pages3
JournalScience
Volume299
Issue number5606
DOIs
StatePublished - Jan 24 2003
Externally publishedYes

ASJC Scopus subject areas

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