Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB

H. Asahara, B. Santoso, E. Guzman, K. Du, P. A. Cole, I. Davidson, M. Montminy

Research output: Contribution to journalArticle

Abstract

The cyclic AMP (cAMP)-responsive factor CREB induces target gene expression via constitutive (Q2) and inducible (KID, for kinase-inducible domain) activation domains that function synergistically in response to cellular signals. KID stimulates transcription via a phospho (Ser133)-dependent interaction with the coactivator paralogs CREB binding protein and p300, whereas Q2 recruits the TFIID complex via a direct association with hTAFII130. Here we investigate the mechanism underlying cooperativity between the Q2 domain and KID in CREB by in vitro transcription assay with naked DNA and chromatin templates containing the cAMP-responsive somatostatin promoter. The Q2 domain was highly active on a naked DNA template, and Ser133 phosphorylation had no additional effect on transcriptional initiation in crude extracts. Q2 activity was repressed on a chromatin template, however, and this repression was relieved by the phospho (Ser133) KID-dependent recruitment of p300 histone acetyltransferase activity to the promoter. In chromatin immunoprecipitation assays of NIH 3T3 cells, cAMP-dependent recruitment of p300 to the somatostatin promoter stimulated acetylation of histone H4. Correspondingly, overexpression of hTAFII130 potentiated CREB activity in cells exposed to cAMP, but had no effect on reporter gene expression in unstimulated cells. We propose that cooperativity between the KID and Q2 domains proceeds via a chromatin-dependent mechanism in which recruitment of p300 facilitates subsequent interaction of CREB with TFIID.

Original languageEnglish (US)
Pages (from-to)7892-7900
Number of pages9
JournalMolecular and Cellular Biology
Volume21
Issue number23
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Chromatin
Phosphotransferases
Cyclic AMP
Transcription Factor TFIID
Somatostatin
CREB-Binding Protein
Histone Acetyltransferases
Gene Expression
NIH 3T3 Cells
Chromatin Immunoprecipitation
DNA
Acetylation
Complex Mixtures
Reporter Genes
Histones
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Asahara, H., Santoso, B., Guzman, E., Du, K., Cole, P. A., Davidson, I., & Montminy, M. (2001). Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. Molecular and Cellular Biology, 21(23), 7892-7900. https://doi.org/10.1128/MCB.21.23.7892-7900.2001

Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. / Asahara, H.; Santoso, B.; Guzman, E.; Du, K.; Cole, P. A.; Davidson, I.; Montminy, M.

In: Molecular and Cellular Biology, Vol. 21, No. 23, 2001, p. 7892-7900.

Research output: Contribution to journalArticle

Asahara, H, Santoso, B, Guzman, E, Du, K, Cole, PA, Davidson, I & Montminy, M 2001, 'Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB', Molecular and Cellular Biology, vol. 21, no. 23, pp. 7892-7900. https://doi.org/10.1128/MCB.21.23.7892-7900.2001
Asahara, H. ; Santoso, B. ; Guzman, E. ; Du, K. ; Cole, P. A. ; Davidson, I. ; Montminy, M. / Chromatin-dependent cooperativity between constitutive and inducible activation domains in CREB. In: Molecular and Cellular Biology. 2001 ; Vol. 21, No. 23. pp. 7892-7900.
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