Cholinergic transmission regulates extrajunctional acetylcholine receptors

Alan Pestronk; Daniel B. Drachman; Elis F. Stanley; Donald L. Price; John W. Griffin

doi:10.1016/0014-4886(80)90193-4

Cholinergic transmission regulates extrajunctional acetylcholine receptors

Alan Pestronk, Daniel B. Drachman, Elis F. Stanley, Donald L. Price, John W. Griffin

School of Medicine

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

To determine the role of ACh transmission in the regulation of extrajunctional ACh receptors, we compared the effect of postsynaptic cholinergic blockade with that of surgical denervation. Blockade of ACh transmission was produced in the soleus muscles of rats by continuous local infusion of α-bungarotoxin, delivered by implantable osmotic pumps. Extrajunctional ACh receptors were measured by an ¹²⁵I-α-BuTx binding method. Our results showed an increase of extrajunctional ACh receptors quantitatively equivalent to that resulting from surgical denervation. This denervation-like effect is attributed to elimination of (i) impulse-dependent ACh transmission (which normally triggers muscle usage), and (ii) spontaneous quantal and nonquantal ACh transmission. The influence of the nerve in regulating extrajunctional ACh receptors appears to be due to the sum of these forms of ACh transmission.

Original language	English (US)
Pages (from-to)	690-696
Number of pages	7
Journal	Experimental Neurology
Volume	70
Issue number	3
DOIs	https://doi.org/10.1016/0014-4886(80)90193-4
State	Published - Dec 1980

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/0014-4886(80)90193-4

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@article{2bccbd83ec2a4bad8ee3fec90f779cdf,

title = "Cholinergic transmission regulates extrajunctional acetylcholine receptors",

abstract = "To determine the role of ACh transmission in the regulation of extrajunctional ACh receptors, we compared the effect of postsynaptic cholinergic blockade with that of surgical denervation. Blockade of ACh transmission was produced in the soleus muscles of rats by continuous local infusion of α-bungarotoxin, delivered by implantable osmotic pumps. Extrajunctional ACh receptors were measured by an 125I-α-BuTx binding method. Our results showed an increase of extrajunctional ACh receptors quantitatively equivalent to that resulting from surgical denervation. This denervation-like effect is attributed to elimination of (i) impulse-dependent ACh transmission (which normally triggers muscle usage), and (ii) spontaneous quantal and nonquantal ACh transmission. The influence of the nerve in regulating extrajunctional ACh receptors appears to be due to the sum of these forms of ACh transmission.",

author = "Alan Pestronk and Drachman, {Daniel B.} and Stanley, {Elis F.} and Price, {Donald L.} and Griffin, {John W.}",

note = "Funding Information: Abbreviations: ACh-acetylcholine, cy-BuTx-o-bungarotoxin. {\textquoteright} We are grateful to Robert N. Adams, Kenneth Fahnestock, and Julienne Tolmasoff for technical assistance and to C. F. Barlow for assistance with the manuscript. This work was supported by grants 5POl NS10920 and 5ROl HDO4817 from the National Institutes of Health. A.P. was supported by a Teacher-Investigator Award from the National Institutes of Health.",

year = "1980",

month = dec,

doi = "10.1016/0014-4886(80)90193-4",

language = "English (US)",

volume = "70",

pages = "690--696",

journal = "Experimental Neurology",

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T1 - Cholinergic transmission regulates extrajunctional acetylcholine receptors

AU - Pestronk, Alan

AU - Drachman, Daniel B.

AU - Stanley, Elis F.

AU - Price, Donald L.

AU - Griffin, John W.

N1 - Funding Information: Abbreviations: ACh-acetylcholine, cy-BuTx-o-bungarotoxin. ’ We are grateful to Robert N. Adams, Kenneth Fahnestock, and Julienne Tolmasoff for technical assistance and to C. F. Barlow for assistance with the manuscript. This work was supported by grants 5POl NS10920 and 5ROl HDO4817 from the National Institutes of Health. A.P. was supported by a Teacher-Investigator Award from the National Institutes of Health.

PY - 1980/12

Y1 - 1980/12

N2 - To determine the role of ACh transmission in the regulation of extrajunctional ACh receptors, we compared the effect of postsynaptic cholinergic blockade with that of surgical denervation. Blockade of ACh transmission was produced in the soleus muscles of rats by continuous local infusion of α-bungarotoxin, delivered by implantable osmotic pumps. Extrajunctional ACh receptors were measured by an 125I-α-BuTx binding method. Our results showed an increase of extrajunctional ACh receptors quantitatively equivalent to that resulting from surgical denervation. This denervation-like effect is attributed to elimination of (i) impulse-dependent ACh transmission (which normally triggers muscle usage), and (ii) spontaneous quantal and nonquantal ACh transmission. The influence of the nerve in regulating extrajunctional ACh receptors appears to be due to the sum of these forms of ACh transmission.

AB - To determine the role of ACh transmission in the regulation of extrajunctional ACh receptors, we compared the effect of postsynaptic cholinergic blockade with that of surgical denervation. Blockade of ACh transmission was produced in the soleus muscles of rats by continuous local infusion of α-bungarotoxin, delivered by implantable osmotic pumps. Extrajunctional ACh receptors were measured by an 125I-α-BuTx binding method. Our results showed an increase of extrajunctional ACh receptors quantitatively equivalent to that resulting from surgical denervation. This denervation-like effect is attributed to elimination of (i) impulse-dependent ACh transmission (which normally triggers muscle usage), and (ii) spontaneous quantal and nonquantal ACh transmission. The influence of the nerve in regulating extrajunctional ACh receptors appears to be due to the sum of these forms of ACh transmission.

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Cholinergic transmission regulates extrajunctional acetylcholine receptors

Abstract

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