TY - JOUR
T1 - Cholinergic neuronal differentiation of bone marrow mesenchymal stem cells in rhesus monkeys
AU - Qi, Ying
AU - Zhang, Feng Yan
AU - Song, Ge
AU - Sun, Xue Rong
AU - Jiang, Ru Zhang
AU - Chen, Meng Fei
AU - Ge, Jian
N1 - Funding Information:
This work was supported by the National Key Basic Research Program of China (Grant No.2007CB512207) and the National Natural Science Foundation of China (Grant No. 30600695/C030309).
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - The purpose of the present study was to determine the best cholinergic neuronal differentiation method of rhesus monkey bone marrow mesenchymal stem cells (BMSCs). Four methods were used to induce differentiation, and the groups were assigned accordingly: basal inducing group (culture media, bFGF, and forskolin); SHH inducing group (SHH, inducing group); RA inducing group (RA, basal inducing group); and SHH+RA inducing group (SHH, RA, and basal inducing group). All groups displayed neuronal morphology and increased expression of nestin and neuron-specific enolase. The basal inducing group did not express synapsin, and cells from the SHH inducing group did not exhibit neuronal resting membrane potential. In contrast, results demonstrated that BMSCs from the RA and SHH+RA inducing groups exhibited neuronal resting membrane potential, and cells from the SHH+RA inducing group expressed higher levels of synapsin and acetylcholine. In conclusion, the induction of cholinergic differentiation through SHH+RA was determined to be superior to the other methods.
AB - The purpose of the present study was to determine the best cholinergic neuronal differentiation method of rhesus monkey bone marrow mesenchymal stem cells (BMSCs). Four methods were used to induce differentiation, and the groups were assigned accordingly: basal inducing group (culture media, bFGF, and forskolin); SHH inducing group (SHH, inducing group); RA inducing group (RA, basal inducing group); and SHH+RA inducing group (SHH, RA, and basal inducing group). All groups displayed neuronal morphology and increased expression of nestin and neuron-specific enolase. The basal inducing group did not express synapsin, and cells from the SHH inducing group did not exhibit neuronal resting membrane potential. In contrast, results demonstrated that BMSCs from the RA and SHH+RA inducing groups exhibited neuronal resting membrane potential, and cells from the SHH+RA inducing group expressed higher levels of synapsin and acetylcholine. In conclusion, the induction of cholinergic differentiation through SHH+RA was determined to be superior to the other methods.
KW - Acetylcholine
KW - Bone marrow mesenchymal stem cells
KW - Neuronal differentiation
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U2 - 10.1007/s11427-010-0009-4
DO - 10.1007/s11427-010-0009-4
M3 - Article
C2 - 20596940
AN - SCOPUS:77953727614
SN - 1674-7305
VL - 53
SP - 573
EP - 580
JO - Science China Life Sciences
JF - Science China Life Sciences
IS - 5
ER -