Cholinergic neuronal differentiation of bone marrow mesenchymal stem cells in rhesus monkeys

Ying Qi, Feng Yan Zhang, Ge Song, Xue Rong Sun, Ru Zhang Jiang, Meng Fei Chen, Jian Ge

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of the present study was to determine the best cholinergic neuronal differentiation method of rhesus monkey bone marrow mesenchymal stem cells (BMSCs). Four methods were used to induce differentiation, and the groups were assigned accordingly: basal inducing group (culture media, bFGF, and forskolin); SHH inducing group (SHH, inducing group); RA inducing group (RA, basal inducing group); and SHH+RA inducing group (SHH, RA, and basal inducing group). All groups displayed neuronal morphology and increased expression of nestin and neuron-specific enolase. The basal inducing group did not express synapsin, and cells from the SHH inducing group did not exhibit neuronal resting membrane potential. In contrast, results demonstrated that BMSCs from the RA and SHH+RA inducing groups exhibited neuronal resting membrane potential, and cells from the SHH+RA inducing group expressed higher levels of synapsin and acetylcholine. In conclusion, the induction of cholinergic differentiation through SHH+RA was determined to be superior to the other methods.

Original languageEnglish (US)
Pages (from-to)573-580
Number of pages8
JournalScience China Life Sciences
Volume53
Issue number5
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Acetylcholine
  • Bone marrow mesenchymal stem cells
  • Neuronal differentiation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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