Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses

Stuart L. Johnson, Carolina Wedemeyer, Douglas E. Vetter, Roberto Adachi, Matthew C. Holley, Ana Belen Elgoyhen, Walter Marcotti

Research output: Contribution to journalArticle

Abstract

Spontaneous electrical activity generated by developing sensory cells and neurons is crucial for the maturation of neural circuits. The full maturation of mammalian auditory inner hair cells (IHCs) depends on patterns of spontaneous action potentials during a 'critical period' of development. The intrinsic spiking activity of IHCs can be modulated by inhibitory input from cholinergic efferent fibres descending from the brainstem, which transiently innervate immature IHCs. However, it remains unknown whether this transient efferent input to developing IHCs is required for their functional maturation. We used a mouse model that lacks the a9-nicotinic acetylcholine receptor subunit (a9nAChR) in IHCs and another lacking synaptotagmin-2 in the efferent terminals to remove or reduce efferent input to IHCs, respectively. We found that the efferent system is required for the developmental linearization of the Ca2p-sensitivity of vesicle fusion at IHC ribbon synapses, without affecting their general cell development. This provides the first direct evidence that the efferent system, by modulating IHC electrical activity, is required for the maturation of the IHC synaptic machinery. The central control of sensory cell development is unique among sensory systems.

Original languageEnglish (US)
Article number130163
JournalOpen Biology
Volume3
Issue numberNOV
DOIs
StatePublished - Nov 27 2013
Externally publishedYes

Fingerprint

Inner Auditory Hair Cells
Auditory Hair Cells
Synaptic Transmission
Synapses
Cholinergic Agents
Synaptotagmin II
Cells
Nicotinic Receptors
Linearization
Neurons
Machinery
Fusion reactions
Networks (circuits)
Fibers
Cholinergic Fibers
Sensory Receptor Cells
Action Potentials
Brain Stem

Keywords

  • Calcium current
  • Cochlea
  • Development
  • Efferent system
  • Exocytosis
  • Hair cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology

Cite this

Johnson, S. L., Wedemeyer, C., Vetter, D. E., Adachi, R., Holley, M. C., Elgoyhen, A. B., & Marcotti, W. (2013). Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses. Open Biology, 3(NOV), [130163]. https://doi.org/10.1098/rsob.130163

Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses. / Johnson, Stuart L.; Wedemeyer, Carolina; Vetter, Douglas E.; Adachi, Roberto; Holley, Matthew C.; Elgoyhen, Ana Belen; Marcotti, Walter.

In: Open Biology, Vol. 3, No. NOV, 130163, 27.11.2013.

Research output: Contribution to journalArticle

Johnson, SL, Wedemeyer, C, Vetter, DE, Adachi, R, Holley, MC, Elgoyhen, AB & Marcotti, W 2013, 'Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses', Open Biology, vol. 3, no. NOV, 130163. https://doi.org/10.1098/rsob.130163
Johnson SL, Wedemeyer C, Vetter DE, Adachi R, Holley MC, Elgoyhen AB et al. Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses. Open Biology. 2013 Nov 27;3(NOV). 130163. https://doi.org/10.1098/rsob.130163
Johnson, Stuart L. ; Wedemeyer, Carolina ; Vetter, Douglas E. ; Adachi, Roberto ; Holley, Matthew C. ; Elgoyhen, Ana Belen ; Marcotti, Walter. / Cholinergic efferent synaptic transmission regulates the maturation of auditory hair cell ribbon synapses. In: Open Biology. 2013 ; Vol. 3, No. NOV.
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