Cholesterol screening: Comparative evaluation of on-site and laboratory-based measurements

P. S. Bachorik, R. Rock, T. Cloey, E. Treciak, D. Becker, W. Sigmund

Research output: Contribution to journalArticlepeer-review

Abstract

We measured cholesterol in capillary blood samples from 9683 volunteers over a four-day on-site community screening program, using the 'Reflotron' desk-top analyzer (Boehringer-Mannheim Diagnostics, Indianapolis, IN). We also measured cholesterol in venous blood samples from 3% of those screened (a) with the Reflotron at the screening sites, (b) in a qualified hospital clinical laboratory, and (c) in a Centers for Disease Control standardized lipoprotein research laboratory. The sensitivity (and specificity) of the Reflotron measurements, with use of the lipoprotein laboratory measurements as the point of reference, was 0.95 (0.73) in capillary blood samples and 0.88 (0.93) in venous blood samples, compared with 0.99 (0.87) in the hospital clinical laboratory. The Reflotron measurements correlated less well with the lipoprotein laboratory values in both venous blood (r=0.89) samples than did the clinical laboratory values (r>0.99). Furthermore, the capillary blood measurements averaged 7% higher than venous measurements when both kinds of samples were analyzed in the Reflotron.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalClinical chemistry
Volume36
Issue number2
DOIs
StatePublished - 1990

Keywords

  • Capillary-venous differences
  • reflectance photometry
  • sensitivity
  • specificity
  • variation, source of

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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