Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation

Dawn Johnson, Julie A. Freischlag, Douglas A. Coe, Junaid H. Mudaliar, David K. Traul, Holly Kelly, Lara Hanson, Robert A. Cambria, Gary R. Seabrook, Jonathan B. Towne

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to determine the physiologic effects of cigarette smoke exposure and dietary cholesterol on the availability of nitric oxide in carotid vascular rings. New Zealand white rabbits were placed in an airflow chamber for 3 hr/day over an 8-week period and were exposed to smoke from 600 cigarettes/per day added to the chamber inflow by a robotic smoke generator. New Zealand white rabbits, made hypercholesterolemic, and one group fed a normal diet, were similarly placed in the chamber without exposure to cigarette smoke. In those exposed groups, serum cotinine and cholesterol levels were consistently elevated. After the 8-week period, the carotid arteries were harvested. The vessels were cut into 3-mm rings which were suspended from pressure transducers. The rings were contracted with potassium chloride (KCl) to determine vessel integrity. One ring from each carotid was maximally contracted with 1 x 10-3 molar norepinephrine (NE) while the experimental ring was contracted to 50% of maximum. Relaxation of the rings was achieved by adding incremental doses of acetylcholine. Our results showed that endothelial dysfunction, as measured by acetylcholine- mediated vasorelaxation, occurs in the rabbit carotid artery when exposed to high dietary cholesterol. Cigarette exposure alone in this particular vessel did not result in significant alteration in acetylcholine-mediated vasorelaxation.

Original languageEnglish (US)
Pages (from-to)480-483
Number of pages4
JournalAnnals of Vascular Surgery
Volume13
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Carotid Arteries
Smoke
Tobacco Products
Cholesterol
Acetylcholine
Rabbits
Dietary Cholesterol
Vasodilation
Pressure Transducers
Cotinine
Potassium Chloride
Robotics
Blood Vessels
Norepinephrine
Nitric Oxide
Diet
Serum

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Johnson, D., Freischlag, J. A., Coe, D. A., Mudaliar, J. H., Traul, D. K., Kelly, H., ... Towne, J. B. (1999). Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation. Annals of Vascular Surgery, 13(5), 480-483. https://doi.org/10.1007/s100169900287

Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation. / Johnson, Dawn; Freischlag, Julie A.; Coe, Douglas A.; Mudaliar, Junaid H.; Traul, David K.; Kelly, Holly; Hanson, Lara; Cambria, Robert A.; Seabrook, Gary R.; Towne, Jonathan B.

In: Annals of Vascular Surgery, Vol. 13, No. 5, 1999, p. 480-483.

Research output: Contribution to journalArticle

Johnson, D, Freischlag, JA, Coe, DA, Mudaliar, JH, Traul, DK, Kelly, H, Hanson, L, Cambria, RA, Seabrook, GR & Towne, JB 1999, 'Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation', Annals of Vascular Surgery, vol. 13, no. 5, pp. 480-483. https://doi.org/10.1007/s100169900287
Johnson D, Freischlag JA, Coe DA, Mudaliar JH, Traul DK, Kelly H et al. Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation. Annals of Vascular Surgery. 1999;13(5):480-483. https://doi.org/10.1007/s100169900287
Johnson, Dawn ; Freischlag, Julie A. ; Coe, Douglas A. ; Mudaliar, Junaid H. ; Traul, David K. ; Kelly, Holly ; Hanson, Lara ; Cambria, Robert A. ; Seabrook, Gary R. ; Towne, Jonathan B. / Cholesterol, but not cigarette smoke, decreases rabbit carotid artery relaxation. In: Annals of Vascular Surgery. 1999 ; Vol. 13, No. 5. pp. 480-483.
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