TY - JOUR
T1 - Cholera antitoxin titrations
T2 - A comparative study of fat-cell, ileal-loop, and rabbit-skin assays
AU - Curtin, George T.
AU - Mosley, Wiley H.
AU - Greenough, William B.
N1 - Funding Information:
This investigation was supported in part by research grant no. AI 07625 of the United States-Japan Cooperative Medical Science Program administered by the National Institute of Allergy and Infectious Diseases of the National Institutes of Health, and in part by research agreement no. 196802 with the Cholera Research Laboratory, Dacca, Bangladesh.
Funding Information:
These studies were carried out at the Cholera Research Laboratory, which is a part of the Cholera Research Program supported by the United States Agency for International Development, Department of State; the National Institutes of Health; the Center for Disease Control; and by the governments of Pakistan, the United Kingdom, and other SEATO nations. The NIH Cholera Advisory Committee coordinates the research program.
PY - 1973/3
Y1 - 1973/3
N2 - The levels of serum antibodies to cholera toxin can be measured by any of three test systems. Each of these assays (isolated fat cell, rabbit skin, and ileal loop) give similar values although there are differences in sensitivity among the systems. The variety of antisera and toxins tested makes it very likely that a single antigen- antibody reaction is being measured by each assay method. Recent proof of the hypothesis that cholera toxin acts by raising levels of cyclic adenosine-3',5'-mono- phosphate in tissues exposed to toxin may explain how the apparent diversity of biological end points observed in the respective test systems may be achieved through a common mechanism of action. It is likely that direct enzymatic methods can be developed to speed and simplify further the assay of this toxin.
AB - The levels of serum antibodies to cholera toxin can be measured by any of three test systems. Each of these assays (isolated fat cell, rabbit skin, and ileal loop) give similar values although there are differences in sensitivity among the systems. The variety of antisera and toxins tested makes it very likely that a single antigen- antibody reaction is being measured by each assay method. Recent proof of the hypothesis that cholera toxin acts by raising levels of cyclic adenosine-3',5'-mono- phosphate in tissues exposed to toxin may explain how the apparent diversity of biological end points observed in the respective test systems may be achieved through a common mechanism of action. It is likely that direct enzymatic methods can be developed to speed and simplify further the assay of this toxin.
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U2 - 10.1093/infdis/127.3.294
DO - 10.1093/infdis/127.3.294
M3 - Article
C2 - 4734668
AN - SCOPUS:0015589323
VL - 127
SP - 294
EP - 298
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 3
ER -