Cholecystectomy alters the hormonal response of the sphincter of Oddi

Pierce A. Grace, Henry A. Pitt

Research output: Contribution to journalArticle

Abstract

We have previously described a cholecysto-sphincter of Oddi reflex whereby sphincter of Oddi (SO) motility is mediated in part by the degree of gallbladder distension. Therefore, we tested the hypothesis that cholecystectomy alters the response of the SO to endogenous and exogenous hormonal stimulation. Eight months after sham laparotomy (n = 8) or cholecystectomy (n = 10), prairie dogs were anesthetized with α-chloralose. The common bile duct was cannulated distally with a side-hole, pressure-monitored catheter perfused with degassed water at 0.15 ml/min. The duodenum was cannulated distal to the SO to allow perfusion of the proximal 30 cm of intestine with 20 mmol/L sodium oleate at 0.4 ml/min. In animals undergoing sham laparotomy the gallbladder was cannulated, aspirated, and kept empty throughout the experiment. SO phasic wave frequency (F), amplitude (A), and baseline pressure were measured for 60 minutes before and during intraduodenal (ID) perfusion of sodium oleate and then for 60 minutes before and 30 minutes during intravenous (IV) infusion of cholecystokinin-octapeptide (CCK-OP) at 10 ng/kg/min. A SO motility index (MI) (MI = F × A) was calculated for each 10-minute period. Common duct diameter and resting SO motility were unaltered 8 months after cholecystectomy. In animals that had sham laparotomy ID infusion of sodium oleate reduced SO MI by 46% (p = 0.06) and 75% (p <0.05) at 30 and 60 minutes, respectively, whereas in animals that had cholecystectomy the reduction in SO MI was only 6% and 25% (p <0.05) during the same periods, In animals that had sham laparotomy IV CCK-OP increased the SO MI by 175% (p <0.05), but in the animals that had cholecystectomy IV CCK-OP increased SO MI by only 60% (no significance). These findings indicate that after cholecystectomy resting SO motility is unaltered, but the response to ID sodium oleate and to IV cholecystokinin is blunted. We suggest that cholecystectomy alters neural pathways that mediate the normal response of the SO to endogenous and exogenous hormonal stimulation.

Original languageEnglish (US)
Pages (from-to)186-194
Number of pages9
JournalSurgery
Volume102
Issue number2
StatePublished - 1987

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Sphincter of Oddi
Cholecystectomy
Sincalide
Laparotomy
Gallbladder
Perfusion
Pressure
Neural Pathways
Sciuridae
Chloralose
Cholecystokinin
Common Bile Duct
Duodenum
Intravenous Infusions
Intestines

ASJC Scopus subject areas

  • Surgery

Cite this

Grace, P. A., & Pitt, H. A. (1987). Cholecystectomy alters the hormonal response of the sphincter of Oddi. Surgery, 102(2), 186-194.

Cholecystectomy alters the hormonal response of the sphincter of Oddi. / Grace, Pierce A.; Pitt, Henry A.

In: Surgery, Vol. 102, No. 2, 1987, p. 186-194.

Research output: Contribution to journalArticle

Grace, PA & Pitt, HA 1987, 'Cholecystectomy alters the hormonal response of the sphincter of Oddi', Surgery, vol. 102, no. 2, pp. 186-194.
Grace, Pierce A. ; Pitt, Henry A. / Cholecystectomy alters the hormonal response of the sphincter of Oddi. In: Surgery. 1987 ; Vol. 102, No. 2. pp. 186-194.
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abstract = "We have previously described a cholecysto-sphincter of Oddi reflex whereby sphincter of Oddi (SO) motility is mediated in part by the degree of gallbladder distension. Therefore, we tested the hypothesis that cholecystectomy alters the response of the SO to endogenous and exogenous hormonal stimulation. Eight months after sham laparotomy (n = 8) or cholecystectomy (n = 10), prairie dogs were anesthetized with α-chloralose. The common bile duct was cannulated distally with a side-hole, pressure-monitored catheter perfused with degassed water at 0.15 ml/min. The duodenum was cannulated distal to the SO to allow perfusion of the proximal 30 cm of intestine with 20 mmol/L sodium oleate at 0.4 ml/min. In animals undergoing sham laparotomy the gallbladder was cannulated, aspirated, and kept empty throughout the experiment. SO phasic wave frequency (F), amplitude (A), and baseline pressure were measured for 60 minutes before and during intraduodenal (ID) perfusion of sodium oleate and then for 60 minutes before and 30 minutes during intravenous (IV) infusion of cholecystokinin-octapeptide (CCK-OP) at 10 ng/kg/min. A SO motility index (MI) (MI = F × A) was calculated for each 10-minute period. Common duct diameter and resting SO motility were unaltered 8 months after cholecystectomy. In animals that had sham laparotomy ID infusion of sodium oleate reduced SO MI by 46{\%} (p = 0.06) and 75{\%} (p <0.05) at 30 and 60 minutes, respectively, whereas in animals that had cholecystectomy the reduction in SO MI was only 6{\%} and 25{\%} (p <0.05) during the same periods, In animals that had sham laparotomy IV CCK-OP increased the SO MI by 175{\%} (p <0.05), but in the animals that had cholecystectomy IV CCK-OP increased SO MI by only 60{\%} (no significance). These findings indicate that after cholecystectomy resting SO motility is unaltered, but the response to ID sodium oleate and to IV cholecystokinin is blunted. We suggest that cholecystectomy alters neural pathways that mediate the normal response of the SO to endogenous and exogenous hormonal stimulation.",
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