Chloride transport across the basolateral cell membrane of the Necturus proximal tubule: Dependence on bicarbonate and sodium

W. B. Guggino, R. London, E. L. Boulpaep, G. Giebisch

Research output: Contribution to journalArticlepeer-review

Abstract

The transport of chloride across the Necturus proximal tubule cell was studied in the doubly-perfused kidney using conventional, chloride-sensitive and pH-sensitive microelectrodes. Lowering chloride activity in the basolateral solution results in a reduction in intracellular Cl- activity (aCli). This reduction in aCli is inhibited by removing either HCO3- or Na+ from the perfusion solution, indicating that both HCO3- and Na+ are required for Cl- movement across the basolateral cell membrane. Reducing either HCO3- or Na+ in the basolateral solution causes an increase in aCli. Thus changes in either Na+ or HCO3- chemical gradients across the basolateral cell membrane significantly affect chloride movement. Changing intracellular pH by means of NH4Cl exposure results in an increase in aCli followed by a sharp decrease when NH4Cl is removed. These changes in intracellular chloride do not occur in the absence of HCO3-. Likewise, the decrease in aCli following NH4Cl treatment requires the presence of Na+ in the basolateral solution. We conclude that chloride is transported across the basolateral cell membrane in exchange for both Na+ and HCO3-. Our results also support the presence of a Na+/Cl- contransport mechanism on the apical cell membrane.

Original languageEnglish (US)
Pages (from-to)227-240
Number of pages14
JournalThe Journal of Membrane Biology
Volume71
Issue number3
DOIs
StatePublished - Oct 1 1983
Externally publishedYes

Keywords

  • Necturus proximal tubule
  • basolateral chloride transport

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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