Chimerism analysis following hematopoietic stem cell transplantation

Kathleen M. Murphy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Allogeneic hematopoietic stem cell transplantation (HSCT) is an effective therapeutic approach for several hematological diseases. Chimerism studies can be helpful to assess donor engraftment, detect early signs of graft rejection, and monitor minimal residual disease. Currently the most common method for monitoring chimerism following HSCT is by PCR amplification of STR loci followed by capillary electrophoresis. Prior to transplantation, multiple STR loci in both the donor and recipient are analyzed in order to identify loci that differentiate the two individuals. Informative loci are selected to calculate the percent donor and recipient present in post-transplant specimens. This is a rapid, sensitive, and cost-effective method for monitoring chimerism in patients following HSCT.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages137-149
Number of pages13
Volume999
DOIs
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume999
ISSN (Print)10643745

Keywords

  • Capillary electrophoresis
  • Engraftment
  • Hematopoietic stem cell transplantation
  • Short tandem repeat (STR)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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