TY - CHAP
T1 - Chimerism analysis following hematopoietic stem cell transplantation
AU - Murphy, Kathleen M.
PY - 2013
Y1 - 2013
N2 - Allogeneic hematopoietic stem cell transplantation (HSCT) is an effective therapeutic approach for several hematological diseases. Chimerism studies can be helpful to assess donor engraftment, detect early signs of graft rejection, and monitor minimal residual disease. Currently the most common method for monitoring chimerism following HSCT is by PCR amplification of STR loci followed by capillary electrophoresis. Prior to transplantation, multiple STR loci in both the donor and recipient are analyzed in order to identify loci that differentiate the two individuals. Informative loci are selected to calculate the percent donor and recipient present in post-transplant specimens. This is a rapid, sensitive, and cost-effective method for monitoring chimerism in patients following HSCT.
AB - Allogeneic hematopoietic stem cell transplantation (HSCT) is an effective therapeutic approach for several hematological diseases. Chimerism studies can be helpful to assess donor engraftment, detect early signs of graft rejection, and monitor minimal residual disease. Currently the most common method for monitoring chimerism following HSCT is by PCR amplification of STR loci followed by capillary electrophoresis. Prior to transplantation, multiple STR loci in both the donor and recipient are analyzed in order to identify loci that differentiate the two individuals. Informative loci are selected to calculate the percent donor and recipient present in post-transplant specimens. This is a rapid, sensitive, and cost-effective method for monitoring chimerism in patients following HSCT.
KW - Capillary electrophoresis
KW - Engraftment
KW - Hematopoietic stem cell transplantation
KW - Short tandem repeat (STR)
UR - http://www.scopus.com/inward/record.url?scp=84883228174&partnerID=8YFLogxK
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U2 - 10.1007/978-1-62703-357-2-9
DO - 10.1007/978-1-62703-357-2-9
M3 - Chapter
C2 - 23666695
AN - SCOPUS:84883228174
SN - 9781627033565
VL - 999
T3 - Methods in Molecular Biology
SP - 137
EP - 149
BT - Methods in Molecular Biology
ER -