Chimeric Na+/H+ exchangers: An epithelial membrane-bound N-terminal domain requires an epithelial cytoplasmic C-terminal domain for regulation by protein kinases

C. H Chris Yun, Chung Ming Tse, Mark Donowitz

Research output: Contribution to journalArticle

Abstract

All cloned members of the mammalian Na+/H+ exchanger gene family encode proteins that consist of two functionally distinct domains: a membrane-bound N terminus and a cytoplasmic C terminus, which are required for ion transport and regulation of transport, respectively. Despite their similarity in structure, three members of this family, designated NHE1, NHE2, and NHE3, exhibit different kinetic mechanisms in response to growth factors and protein kinases. For instance, growth factors stimulate NHE1 by a change in the affinity constant for intracellular H+, K'(H(i)/+), and regulate NHE2 and NHE3 by a change in V(max). We have constructed chimeric Na+/H+ exchangers by exchanging the N and C termini among three cloned rabbit Na+/H+ exchangers (NHE1 to NHE3) to determine which domain is responsible for the above V(max)-vs.-K'(H(i)/+) effect of the Na+/H+ isoforms. All of the chimeras had functional exchange activity and basal kinetic properties similar to those of wild-type exchangers. Studies with serum showed that the N terminus is responsible for the V(max)-vs.-K'(H(i)/+) stimulation of the Na+/H- exchanger isoforms. Moreover, phorbol 12-myristate 13-acetate and fibroblast growth factor altered Na+/H+ exchange only in chimeras that had an epithelial N-terminal domain Matched with an epithelial C-terminal domain. Therefore, the protein kinase-induced regulation of Na+/H+ exchangers is mediated through a specific interaction between the N- and C-termini, which is restricted so that epithelial N- and epithelial C-terminal portions of the exchangers are required for regulation.

Original languageEnglish (US)
Pages (from-to)10723-10727
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number23
DOIs
StatePublished - Nov 7 1995

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Sodium-Hydrogen Antiporter
Protein Kinases
Membranes
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Ion Transport
Acetates
Rabbits
Serum
Genes
Proteins

Keywords

  • Na absorption
  • Na-H antiporter
  • pH regulation
  • signal transduction

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

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title = "Chimeric Na+/H+ exchangers: An epithelial membrane-bound N-terminal domain requires an epithelial cytoplasmic C-terminal domain for regulation by protein kinases",
abstract = "All cloned members of the mammalian Na+/H+ exchanger gene family encode proteins that consist of two functionally distinct domains: a membrane-bound N terminus and a cytoplasmic C terminus, which are required for ion transport and regulation of transport, respectively. Despite their similarity in structure, three members of this family, designated NHE1, NHE2, and NHE3, exhibit different kinetic mechanisms in response to growth factors and protein kinases. For instance, growth factors stimulate NHE1 by a change in the affinity constant for intracellular H+, K'(H(i)/+), and regulate NHE2 and NHE3 by a change in V(max). We have constructed chimeric Na+/H+ exchangers by exchanging the N and C termini among three cloned rabbit Na+/H+ exchangers (NHE1 to NHE3) to determine which domain is responsible for the above V(max)-vs.-K'(H(i)/+) effect of the Na+/H+ isoforms. All of the chimeras had functional exchange activity and basal kinetic properties similar to those of wild-type exchangers. Studies with serum showed that the N terminus is responsible for the V(max)-vs.-K'(H(i)/+) stimulation of the Na+/H- exchanger isoforms. Moreover, phorbol 12-myristate 13-acetate and fibroblast growth factor altered Na+/H+ exchange only in chimeras that had an epithelial N-terminal domain Matched with an epithelial C-terminal domain. Therefore, the protein kinase-induced regulation of Na+/H+ exchangers is mediated through a specific interaction between the N- and C-termini, which is restricted so that epithelial N- and epithelial C-terminal portions of the exchangers are required for regulation.",
keywords = "Na absorption, Na-H antiporter, pH regulation, signal transduction",
author = "Yun, {C. H Chris} and Tse, {Chung Ming} and Mark Donowitz",
year = "1995",
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T1 - Chimeric Na+/H+ exchangers

T2 - An epithelial membrane-bound N-terminal domain requires an epithelial cytoplasmic C-terminal domain for regulation by protein kinases

AU - Yun, C. H Chris

AU - Tse, Chung Ming

AU - Donowitz, Mark

PY - 1995/11/7

Y1 - 1995/11/7

N2 - All cloned members of the mammalian Na+/H+ exchanger gene family encode proteins that consist of two functionally distinct domains: a membrane-bound N terminus and a cytoplasmic C terminus, which are required for ion transport and regulation of transport, respectively. Despite their similarity in structure, three members of this family, designated NHE1, NHE2, and NHE3, exhibit different kinetic mechanisms in response to growth factors and protein kinases. For instance, growth factors stimulate NHE1 by a change in the affinity constant for intracellular H+, K'(H(i)/+), and regulate NHE2 and NHE3 by a change in V(max). We have constructed chimeric Na+/H+ exchangers by exchanging the N and C termini among three cloned rabbit Na+/H+ exchangers (NHE1 to NHE3) to determine which domain is responsible for the above V(max)-vs.-K'(H(i)/+) effect of the Na+/H+ isoforms. All of the chimeras had functional exchange activity and basal kinetic properties similar to those of wild-type exchangers. Studies with serum showed that the N terminus is responsible for the V(max)-vs.-K'(H(i)/+) stimulation of the Na+/H- exchanger isoforms. Moreover, phorbol 12-myristate 13-acetate and fibroblast growth factor altered Na+/H+ exchange only in chimeras that had an epithelial N-terminal domain Matched with an epithelial C-terminal domain. Therefore, the protein kinase-induced regulation of Na+/H+ exchangers is mediated through a specific interaction between the N- and C-termini, which is restricted so that epithelial N- and epithelial C-terminal portions of the exchangers are required for regulation.

AB - All cloned members of the mammalian Na+/H+ exchanger gene family encode proteins that consist of two functionally distinct domains: a membrane-bound N terminus and a cytoplasmic C terminus, which are required for ion transport and regulation of transport, respectively. Despite their similarity in structure, three members of this family, designated NHE1, NHE2, and NHE3, exhibit different kinetic mechanisms in response to growth factors and protein kinases. For instance, growth factors stimulate NHE1 by a change in the affinity constant for intracellular H+, K'(H(i)/+), and regulate NHE2 and NHE3 by a change in V(max). We have constructed chimeric Na+/H+ exchangers by exchanging the N and C termini among three cloned rabbit Na+/H+ exchangers (NHE1 to NHE3) to determine which domain is responsible for the above V(max)-vs.-K'(H(i)/+) effect of the Na+/H+ isoforms. All of the chimeras had functional exchange activity and basal kinetic properties similar to those of wild-type exchangers. Studies with serum showed that the N terminus is responsible for the V(max)-vs.-K'(H(i)/+) stimulation of the Na+/H- exchanger isoforms. Moreover, phorbol 12-myristate 13-acetate and fibroblast growth factor altered Na+/H+ exchange only in chimeras that had an epithelial N-terminal domain Matched with an epithelial C-terminal domain. Therefore, the protein kinase-induced regulation of Na+/H+ exchangers is mediated through a specific interaction between the N- and C-termini, which is restricted so that epithelial N- and epithelial C-terminal portions of the exchangers are required for regulation.

KW - Na absorption

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KW - pH regulation

KW - signal transduction

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JO - Proceedings of the National Academy of Sciences of the United States of America

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