Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite

Thomas G P Grünewald, Virginie Bernard, Pascale Gilardi-Hebenstreit, Virginie Raynal, Didier Surdez, Marie Ming Aynaud, Olivier Mirabeau, Florencia Cidre-Aranaz, Franck Tirode, Sakina Zaidi, Gaëlle Perot, Anneliene H. Jonker, Carlo Lucchesi, Marie Cécile Le Deley, Odile Oberlin, Perrine Marec-Bérard, Amélie S. Véron, Stephanie Reynaud, Eve Lapouble, Valentina BoevaThomas Rio Frio, Javier Alonso, Smita Bhatia, Gaëlle Pierron, Geraldine Cancel-Tassin, Olivier Cussenot, David G. Cox, Lindsay M. Morton, Mitchell J. MacHiela, Stephen J. Chanock, Patrick Charnay, Olivier Delattre

Research output: Contribution to journalArticle

Abstract

Deciphering the ways in which somatic mutations and germline susceptibility variants cooperate to promote cancer is challenging. Ewing sarcoma is characterized by fusions between EWSR1 and members of the ETS gene family, usually EWSR1-FLI1, leading to the generation of oncogenic transcription factors that bind DNA at GGAA motifs. A recent genome-wide association study identified susceptibility variants near EGR2. Here we found that EGR2 knockdown inhibited proliferation, clonogenicity and spheroidal growth in vitro and induced regression of Ewing sarcoma xenografts. Targeted germline deep sequencing of the EGR2 locus in affected subjects and controls identified 291 Ewing-associated SNPs. At rs79965208, the A risk allele connected adjacent GGAA repeats by converting an interspaced GGAT motif into a GGAA motif, thereby increasing the number of consecutive GGAA motifs and thus the EWSR1-FLI1-dependent enhancer activity of this sequence, with epigenetic characteristics of an active regulatory element. EWSR1-FLI1 preferentially bound to the A risk allele, which increased global and allele-specific EGR2 expression. Collectively, our findings establish cooperation between a dominant oncogene and a susceptibility variant that regulates a major driver of Ewing sarcomagenesis.

Original languageEnglish (US)
Pages (from-to)1073-1078
Number of pages6
JournalNature Genetics
Volume47
Issue number9
DOIs
StatePublished - Aug 27 2015
Externally publishedYes

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Ewing's Sarcoma
Microsatellite Repeats
Alleles
Genes
High-Throughput Nucleotide Sequencing
Germ-Line Mutation
Genome-Wide Association Study
Oncogenes
Heterografts
Epigenomics
Single Nucleotide Polymorphism
Transcription Factors
DNA
Growth
Neoplasms

ASJC Scopus subject areas

  • Genetics

Cite this

Grünewald, T. G. P., Bernard, V., Gilardi-Hebenstreit, P., Raynal, V., Surdez, D., Aynaud, M. M., ... Delattre, O. (2015). Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite. Nature Genetics, 47(9), 1073-1078. https://doi.org/10.1038/ng.3363

Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite. / Grünewald, Thomas G P; Bernard, Virginie; Gilardi-Hebenstreit, Pascale; Raynal, Virginie; Surdez, Didier; Aynaud, Marie Ming; Mirabeau, Olivier; Cidre-Aranaz, Florencia; Tirode, Franck; Zaidi, Sakina; Perot, Gaëlle; Jonker, Anneliene H.; Lucchesi, Carlo; Le Deley, Marie Cécile; Oberlin, Odile; Marec-Bérard, Perrine; Véron, Amélie S.; Reynaud, Stephanie; Lapouble, Eve; Boeva, Valentina; Frio, Thomas Rio; Alonso, Javier; Bhatia, Smita; Pierron, Gaëlle; Cancel-Tassin, Geraldine; Cussenot, Olivier; Cox, David G.; Morton, Lindsay M.; MacHiela, Mitchell J.; Chanock, Stephen J.; Charnay, Patrick; Delattre, Olivier.

In: Nature Genetics, Vol. 47, No. 9, 27.08.2015, p. 1073-1078.

Research output: Contribution to journalArticle

Grünewald, TGP, Bernard, V, Gilardi-Hebenstreit, P, Raynal, V, Surdez, D, Aynaud, MM, Mirabeau, O, Cidre-Aranaz, F, Tirode, F, Zaidi, S, Perot, G, Jonker, AH, Lucchesi, C, Le Deley, MC, Oberlin, O, Marec-Bérard, P, Véron, AS, Reynaud, S, Lapouble, E, Boeva, V, Frio, TR, Alonso, J, Bhatia, S, Pierron, G, Cancel-Tassin, G, Cussenot, O, Cox, DG, Morton, LM, MacHiela, MJ, Chanock, SJ, Charnay, P & Delattre, O 2015, 'Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite', Nature Genetics, vol. 47, no. 9, pp. 1073-1078. https://doi.org/10.1038/ng.3363
Grünewald TGP, Bernard V, Gilardi-Hebenstreit P, Raynal V, Surdez D, Aynaud MM et al. Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite. Nature Genetics. 2015 Aug 27;47(9):1073-1078. https://doi.org/10.1038/ng.3363
Grünewald, Thomas G P ; Bernard, Virginie ; Gilardi-Hebenstreit, Pascale ; Raynal, Virginie ; Surdez, Didier ; Aynaud, Marie Ming ; Mirabeau, Olivier ; Cidre-Aranaz, Florencia ; Tirode, Franck ; Zaidi, Sakina ; Perot, Gaëlle ; Jonker, Anneliene H. ; Lucchesi, Carlo ; Le Deley, Marie Cécile ; Oberlin, Odile ; Marec-Bérard, Perrine ; Véron, Amélie S. ; Reynaud, Stephanie ; Lapouble, Eve ; Boeva, Valentina ; Frio, Thomas Rio ; Alonso, Javier ; Bhatia, Smita ; Pierron, Gaëlle ; Cancel-Tassin, Geraldine ; Cussenot, Olivier ; Cox, David G. ; Morton, Lindsay M. ; MacHiela, Mitchell J. ; Chanock, Stephen J. ; Charnay, Patrick ; Delattre, Olivier. / Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite. In: Nature Genetics. 2015 ; Vol. 47, No. 9. pp. 1073-1078.
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abstract = "Deciphering the ways in which somatic mutations and germline susceptibility variants cooperate to promote cancer is challenging. Ewing sarcoma is characterized by fusions between EWSR1 and members of the ETS gene family, usually EWSR1-FLI1, leading to the generation of oncogenic transcription factors that bind DNA at GGAA motifs. A recent genome-wide association study identified susceptibility variants near EGR2. Here we found that EGR2 knockdown inhibited proliferation, clonogenicity and spheroidal growth in vitro and induced regression of Ewing sarcoma xenografts. Targeted germline deep sequencing of the EGR2 locus in affected subjects and controls identified 291 Ewing-associated SNPs. At rs79965208, the A risk allele connected adjacent GGAA repeats by converting an interspaced GGAT motif into a GGAA motif, thereby increasing the number of consecutive GGAA motifs and thus the EWSR1-FLI1-dependent enhancer activity of this sequence, with epigenetic characteristics of an active regulatory element. EWSR1-FLI1 preferentially bound to the A risk allele, which increased global and allele-specific EGR2 expression. Collectively, our findings establish cooperation between a dominant oncogene and a susceptibility variant that regulates a major driver of Ewing sarcomagenesis.",
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AU - Grünewald, Thomas G P

AU - Bernard, Virginie

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AU - Raynal, Virginie

AU - Surdez, Didier

AU - Aynaud, Marie Ming

AU - Mirabeau, Olivier

AU - Cidre-Aranaz, Florencia

AU - Tirode, Franck

AU - Zaidi, Sakina

AU - Perot, Gaëlle

AU - Jonker, Anneliene H.

AU - Lucchesi, Carlo

AU - Le Deley, Marie Cécile

AU - Oberlin, Odile

AU - Marec-Bérard, Perrine

AU - Véron, Amélie S.

AU - Reynaud, Stephanie

AU - Lapouble, Eve

AU - Boeva, Valentina

AU - Frio, Thomas Rio

AU - Alonso, Javier

AU - Bhatia, Smita

AU - Pierron, Gaëlle

AU - Cancel-Tassin, Geraldine

AU - Cussenot, Olivier

AU - Cox, David G.

AU - Morton, Lindsay M.

AU - MacHiela, Mitchell J.

AU - Chanock, Stephen J.

AU - Charnay, Patrick

AU - Delattre, Olivier

PY - 2015/8/27

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